Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method

被引：27

作者：

Abdullah, J. ^{[1
]}

Saffie, N. ^{[1
]}

Sjasri, F. A. R. ^{[1
]}

Husin, A. ^{[3
]}

Abdul-Rahman, Z. ^{[2
]}

Ismail, A. ^{[1
]}

Aziah, I. ^{[1
]}

Mohamed, M. ^{[1
]}

机构：

[1] Inst Res Mol Med, Kubang Kerian, Kelantan, Malaysia

[2] Univ Sains Malaysia, Sch Med Sci, Kubang Kerian, Kelantan, Malaysia

[3] Hosp Raja Perempuan Zainab II, Kota Baharu 15586, Kelantan, Malaysia

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2014年 / 45卷 / 04期

关键词：

loop-mediated isothermal amplification (LAMP); Salmonella Typhi; POLYMERASE-CHAIN-REACTION; REVERSE-TRANSCRIPTION; ENTERICA; SAMPLES; FEVER; ASSAY; FOOD; PCR;

D O I：

10.1590/S1517-83822014000400032

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and -10 times more sensitive in detecting S. Typhi compared to the optimised conventional polymerase chain reaction (PCR) method.

引用

下载

页码：1385 / 1391

页数：7

共 50 条

[1] Loop-Mediated Isothermal Amplification (LAMP) for the Detection of Salmonella in Food
P. A. Kokkinos
P. G. Ziros
M. Bellou
A. Vantarakis
Food Analytical Methods, 2014, 7 : 512 - 526
[2] Loop-Mediated Isothermal Amplification (LAMP) for the Detection of Salmonella in Food
Kokkinos, P. A.
Ziros, P. G.
Bellou, M.
Vantarakis, A.
FOOD ANALYTICAL METHODS, 2014, 7 (02) : 512 - 526
[3] The Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Salmonella enterica serovar Typhi
Fan, Fenxia
Du, Pengcheng
Kan, Biao
Yan, Meiying
PLOS ONE, 2015, 10 (04):
[4] Loop-mediated isothermal amplification for the rapid detection of Salmonella
Hara-Kudo, Y
Yoshino, M
Kojima, T
Ikedo, M
FEMS MICROBIOLOGY LETTERS, 2005, 253 (01) : 155 - 161
[5] The Rapid Detection of Salmonella from Food Samples By Loop-Mediated Isothermal Amplification (LAMP)
Ueda, Shigeko
Kuwabara, Yoshihiro
BIOCONTROL SCIENCE, 2009, 14 (02) : 73 - 76
[6] Rapid detection of mecA and spa by the loop-mediated isothermal amplification (LAMP) method
Koide, Y.
Maeda, H.
Yamabe, K.
Naruishi, K.
Yamamoto, T.
Kokeguchi, S.
Takashiba, S.
LETTERS IN APPLIED MICROBIOLOGY, 2010, 50 (04) : 386 - 392
[7] ESTABLISHMENT OF AN IN-HOUSE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) FOR A RAPID DETECTION OF SALMONELLA TYPHI AND SALMONELLA PARATYPHI A AT LOW-RESOURCE SETTINGS
Saffie, Nur-Eliana
Abdullah, Julia
Rahman, Zaidah A.
Hussin, Azura
Ismail, Asma
Mohamed, Maizan
JOURNAL OF FOOD SAFETY, 2014, 34 (01) : 69 - 75
[8] Rapid detection of Salmonella based on loop-mediated isothermal amplification
Ou, Hongling
Wang, Yan
Gao, Jiayi
Bai, Jing
Zhang, Qiaoyun
Shi, Lei
Wang, Xinru
Wang, Chengbin
ANNALS OF PALLIATIVE MEDICINE, 2021, 10 (06) : 6850 - 6858
[9] Specific and rapid detection of foodborne Salmonella by loop-mediated isothermal amplification method
Wang, Li
Shi, Lei
Alam, M. J.
Geng, Yuhuan
Li, Lin
FOOD RESEARCH INTERNATIONAL, 2008, 41 (01) : 69 - 74
[10] Loop-Mediated Isothermal Amplification (LAMP) for the Rapid and Sensitive Detection of Salmonella Typhimurium from Pork
Techathuvanan, Chayapa
Draughon, Frances Ann
D'Souza, Doris Helen
JOURNAL OF FOOD SCIENCE, 2010, 75 (03) : M165 - M172

← 1 2 3 4 5 →