Analysis of trimethylsilyl derivatization products of phosphatidylethanol by gas chromatography-mass spectrometry

被引：14
|
作者：
Yon, C
Han, JS
机构：
[1] Hanyang Univ, Coll Med, Dept Biochem, Seoul 133792, South Korea
[2] Hanyang Univ, Inst Biomed Sci, Seoul 133792, South Korea
来源：
EXPERIMENTAL AND MOLECULAR MEDICINE | 2000年 / 32卷 / 04期
关键词：
GC-MS; phosphatidylethanol; trimethylsilyl derivative; BSTFA;
D O I：
10.1038/emm.2000.40
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
For the detection of rare phospholipid, phosphatidylethanol (PEt), GC-MS analysis method was adopted for the detection of derivatization products of PR by N,O-bis (trimethylsilyl) trifluroacetamide (BSTFA). A re-structured molecule derived from PEt, ethyl bis (trimethylsilyl)-phosphate was found from search of Wiley database. This molecule can be used as a marker for PR analysis. The molecular formula was C8H23O4PSi2 and weight of the formula was 270.09.
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页码：243 / 245
页数：3
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