The assembly of yeast mitochondrial ATP synthase: subunit depletion in vivo suggests ordered assembly of the stalk subunits b, OSCP and d

被引：24

作者：

Straffon, AFL ^{[1
]}

Prescott, M ^{[1
]}

Nagley, P ^{[1
]}

Devenish, RJ ^{[1
]}

机构：

[1] Monash Univ, Dept Biochem & Mol Biol, Melbourne, Vic 3168, Australia

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1998年 / 1371卷 / 02期

基金：

澳大利亚研究理事会;

关键词：

mitochondrion; ATP synthase; stalk region; assembly; subunit depletion; (yeast);

D O I：

10.1016/S0005-2736(98)00034-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The abundance in vivo of each of three subunits b, OSCP and ct, components of the stalk region of the yeast mitochondrial ATP synthase complex, was manipulated by a controlled depletion strategy. Western blots of whole cell lysates were used to study the effect of depletion of each of these subunits on the cellular levels of other subunits of the enzyme complex. A hierarchy of subunit stability was determined and interpreted to indicate the order of assembly of these three subunits of the stalk region. Thus, subunit b is assembled first, followed by OSCP and then by subunit d. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：157 / 162

页数：6

共 44 条

[31] Mutations in the dimerization domain of the b subunit from the Escherichia coli ATP synthase -: Deletions disrupt function but not enzyme assembly
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[32] Assembly of the stator in Escherichia coli ATP synthase.: Complexation of α subunit with other F1 subunits is prerequisite for δ subunit binding to the n-terminal region of α
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[33] Second stalk composed of the b- and σ-subunits connects F0 to F1 via an α-subunit in the Escherichia coli ATP synthase
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Journal of Biological Chemistry, 1998, 273 (45):
[34] Segregation of b subunits of unequal lengths during assembly of Escherichia coli F1F0 ATP synthase
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[35] The second stalk composed of the b- and δ-subunits connects F0 to F1 via an α-subunit in the Escherichia coli ATP synthase
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[37] T1121G Point Mutation in the Mitochondrial Gene COX1 Suppresses a Null Mutation in ATP23 Required for the Assembly of Yeast Mitochondrial ATP Synthase
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[38] Single copies of subunits d, oligomycin-sensitivity conferring protein, and b are present in the Saccharomyces cerevisiae mitochondrial ATP synthase
J Biol Chem, 2-11 (7462-7466):
[39] Single copies of subunits d, oligomycin-sensitivity conferring protein, and b are present in the Saccharomyces cerevisiae mitochondrial ATP synthase
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[40] Low resolution structure of subunit b (b22–156) of Escherichia coli F1FO ATP synthase in solution and the b−δ assembly
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