Data on charge separation of bispecific and mispaired IgGs using native charge -variant mass spectrometry

被引：5

作者：

Phung, Wilson ^{[1
,2
,3
]}

Han, Guanghui ^{[7
]}

Polderdijk, Stephanie G. I. ^{[4
]}

Dillon, Michael ^{[4
]}

Shatz, Whitney ^{[5
]}

Liu, Peter ^{[1
,2
,3
]}

Wei, Bingchuan ^{[6
]}

Suresh, Pawankumar ^{[6
]}

Fischer, David ^{[6
]}

Spiess, Christoph ^{[4
]}

Bailey, Aaron ^{[7
]}

Carter, Paul J. ^{[4
]}

Lill, Jennie R. ^{[1
,2
,3
]}

Sandoval, Wendy ^{[1
,2
,3
]}

机构：

[1] Genentech Inc, Dept Microchem, 1 DNA Way, San Francisco, CA 94080 USA

[2] Genentech Inc, Dept Prote, 1 DNA Way, San Francisco, CA 94080 USA

[3] Genentech Inc, Dept Lipid, 1 DNA Way, San Francisco, CA 94080 USA

[4] Genentech Inc, Antibody Engn, 1 DNA Way, San Francisco, CA 94080 USA

[5] Genentech Inc, Prot Chem, 1 DNA Way, San Francisco, CA 94080 USA

[6] Genentech Inc, Prot Analyt Chem, 1 DNA Way, San Francisco, CA 94080 USA

[7] BGI Amer, San Jose Mass Spectrometry Ctr, San Jose, CA USA

来源：

DATA IN BRIEF | 2020年 / 30卷

关键词：

Bispecific antibody; Cognate pairs; Single cell; Charge variant mass spectrometry; Ion exchange chromatography; Weak cation exchange; Native mass spectrometry; Molecular model;

D O I：

10.1016/j.dib.2020.105435

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The data supplied in this work are related to the research article entitled "Characterization of Bispecific and Mispaired IgGs by Native Charge-Variant Mass Spectrometry"(Phung et al., 2019). This data article describes a powerful analytical platform using native weak cation exchange chromatography coupled to a high-resolution mass spectrometer, charge variant mass spectrometry (CV-MS), to characterize bispecific and mispaired antibody species. Elution order is investigated through analytical methods and molecular modeling in an effort to understand the intrinsic charge, size and shape differences of these molecules. (C) 2020 The Author(s). Published by Elsevier Inc.

引用

页数：9

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