Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry

被引：225
|
作者：
Kumar, Ravindra
El-Sagheer, Afaf
Tumpane, John
Lincoln, Per
Wilhelmsson, L. Marcus
Brown, Tom [1 ]
机构：
[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England
[2] Chalmers, Dept Chem & Biol Engn Phys Chem, SE-41296 Gothenburg, Sweden
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 21期
关键词：
D O I：
10.1021/ja070273v
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
引用
收藏
页码：6859 / 6864
页数：6
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