Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry

被引：225
|
作者：
Kumar, Ravindra
El-Sagheer, Afaf
Tumpane, John
Lincoln, Per
Wilhelmsson, L. Marcus
Brown, Tom [1 ]
机构：
[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England
[2] Chalmers, Dept Chem & Biol Engn Phys Chem, SE-41296 Gothenburg, Sweden
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 21期
关键词：
D O I：
10.1021/ja070273v
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
引用
收藏
页码：6859 / 6864
页数：6
相关论文
共 38 条
  • [1] Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry
    Kumar, Ravindra
    El-Sagheer, Afaf
    Tumpane, John
    Lincoln, Per
    Wilhelmsson, L. Marcus
    Brown, Tom
    1600, American Chemical Society, 2540 Olentangy River Road, P.O. Box 3337, Columbus, OH 43210-3337, United States (129):
  • [2] OLIGONUCLEOTIDE CIRCULARIZATION BY TEMPLATE-DIRECTED CHEMICAL LIGATION
    DOLINNAYA, NG
    BLUMENFELD, M
    MERENKOVA, IN
    ORETSKAYA, TS
    KRYNETSKAYA, NF
    IVANOVSKAYA, MG
    VASSEUR, M
    SHABAROVA, ZA
    NUCLEIC ACIDS RESEARCH, 1993, 21 (23) : 5403 - 5407
  • [3] TEMPLATE-DIRECTED OLIGONUCLEOTIDE LIGATION ON HYDROXYLAPATITE
    ACEVEDO, OL
    ORGEL, LE
    NATURE, 1986, 321 (6072) : 790 - 792
  • [4] The fidelity of template-directed oligonucleotide ligation and its relevance to DNA computation
    James, KD
    Boles, AR
    Henckel, D
    Ellington, AD
    NUCLEIC ACIDS RESEARCH, 1998, 26 (22) : 5203 - 5211
  • [5] Formation of oligonucleotide-PNA-chimeras by template-directed ligation
    Koppitz, M
    Nielsen, PE
    Orgel, LE
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (19) : 4563 - 4569
  • [6] The fidelity of template-directed oligonucleotide ligation and the inevitability of polymerase function
    James, KD
    Ellington, AD
    ORIGINS OF LIFE AND EVOLUTION OF THE BIOSPHERE, 1999, 29 (04): : 375 - 390
  • [7] The Fidelity of Template-Directed Oligonucleotide Ligation and the Inevitability of Polymerase Function
    Kenneth D. James
    Andrew D. Ellington
    Origins of life and evolution of the biosphere, 1999, 29 : 375 - 390
  • [8] Template-Directed Fluorogenic Oligonucleotide Ligation Using "Click" Chemistry: Detection of Single Nucleotide Polymorphism in the Human p53 Tumor Suppressor Gene
    Sun, Huabing
    Peng, Xiaohua
    BIOCONJUGATE CHEMISTRY, 2013, 24 (07) : 1226 - 1234
  • [9] DNA template-directed oligodeoxynucleotide and PNA crosslinking by strain-promoted click chemistry
    Yue, Xuan
    Fang, Huafeng
    Taylor, John-Stephen
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 231
  • [10] Quantitative Emergence of Hetero[4]rotaxanes by Template-Directed Click Chemistry
    Ke, Chenfeng
    Smaldone, Ronald A.
    Kikuchi, Takashi
    Li, Hao
    Davis, Anthony P.
    Stoddart, J. Fraser
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (01) : 381 - 387
1234