The present study sought to determine the effects of multi-walled carbon nanotubes (MWCNTs) concentrations (0-10 mg L-1) on tetracycline (TC) and biogas slurry nutrient removal by microalga Chlorella vulgaris cultivation. Treatments with 1 mg L-1 MWCNTs yielded the maximum chlorophyll a content, dry weight, and algal density of 143.73 +/- 3.11 mu g L-1, 0.81 +/- 0.008 g L-1, and 5.83x10(7) cells L-1, respectively, suggesting that 1 mg L-1 MWCNTs could enhance microalgal growth performance and photosynthesis effectively. The highest removal rates of chemical oxygen demand, total phosphorus, total nitrogen, TC, and CO2 under 1 mg L-1 MWCNTs were 90.43 +/- 5.15%, 78.12 +/- 4.33%, 77.07 +/- 4.12%, 89.64 +/- 3.08%, and 64.26 +/- 0.71%, respectively. These results elucidated that moderate MWCNTs concentrations might promote TC and nutrient removal by enhancing Chlorella vulgaris photosynthesis activity.