Profiling of Sugar Nucleotides

被引：18
|
作者：
Rejzek, Martin [1 ]
Hill, Lionel [1 ]
Hems, Edward S. [1 ]
Kuhaudomlarp, Sakonwan [1 ]
Wagstaff, Ben A. [1 ]
Field, Robert A. [1 ]
机构：
[1] John Innes Ctr, Norwich, Norfolk, England
来源：
CHEMICAL GLYCOBIOLOGY, PT A: SYNTHESIS, MANIPULATION AND APPLICATIONS OF GLYCANS | 2017年 / 597卷
基金：
英国生物技术与生命科学研究理事会;
关键词：
TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; POROUS GRAPHITIC CARBON; ANION-EXCHANGE CHROMATOGRAPHY; REVERSED-PHASE HPLC; MAMMALIAN-CELLS; PROTEIN GLYCOSYLATION; QUANTITATIVE-ANALYSIS; TRYPANOSOMA-BRUCEI; UDP-GLUCOSE;
D O I：
10.1016/bs.mie.2017.06.005
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Sugar nucleotides are essential building blocks for the glycobiology of all living organisms. Detailed information on the types of sugar nucleotides present in a particular cell and how they change as a function of metabolic, developmental, or disease status is vital. The extraction, identification, and quantification of sugar nucleotides in a given sample present formidable challenges. In this chapter, currently used techniques for sugar nucleotide extraction from cells, separation from complex biological matrices, and detection by optical and mass spectrometry methods are discussed.
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页码：209 / 238
页数：30
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