Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification

被引：14

作者：

He, Peiyan ^{[1
]}

Wang, Henghui ^{[1
]}

Yan, Yong ^{[1
]}

Zhu, Guoying ^{[1
]}

Chen, Zhongwen ^{[1
]}

机构：

[1] Jiaxing Ctr Dis Control & Prevent, 486 Wen Qiao Rd, Jiaxing 314050, Zhejiang, Peoples R China

来源：

JOURNAL OF FLUORESCENCE | 2022年 / 32卷 / 02期

关键词：

Shigella flexneri; Shigella sonnei; Shigella bogdii; Shigella dysenteriae; Multiplex fluorescent PCR; REAL-TIME PCR; ENTEROINVASIVE ESCHERICHIA-COLI; ANTIMICROBIAL RESISTANCE; SPP; DIFFERENTIATION; INFECTIONS; BURDEN; EIEC;

D O I：

10.1007/s10895-021-02876-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was to develop a multiplex fluorescent PCR for Shigella detection and species identification. Five primer pairs for Shigella detection and species identification were designed by Primer Premier 5.0. The multiplex fluorescent PCR was optimized by varying single parameter while other parameters were maintained. The multiplex fluorescent PCR assay could correctly detect Shigella and identify four Shigella species with a detection limits of 10 pg genomic DNA per reaction. Testing different strains and clinical samples confirmed the sensitivity and specificity of the multiplex fluorescent PCR. The newly developed multiplex fluorescent PCR assay is simple, sensitive and specific for Shigella detection and species identification. It has a potential to be used in routine Shigella detection and species identification in clinical laboratories.

引用

页码：707 / 713

页数：7

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