PARTICLE SIZE DETERMINATION IN IMPEDANCE FLOW CYTOMETRY USING MEASURED OPACITY

被引：0

作者：

de Bruijn, Douwe S. ^{[1
]}

Jorissen, Koen F. A. ^{[1
]}

Olthuis, Wouter ^{[1
]}

van den Berg, Albert ^{[1
]}

机构：

[1] Univ Twente, Max Planck Ctr Complex Fluid Dynam, MESA Inst, BIOS Lab Chip Grp, POB 217, NL-7500 AE Enschede, Netherlands

来源：

2021 21ST INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS) | 2021年

关键词：

Flow cytometry; size determination; electrical opacity; microfluidics; coplanar electrodes; POSITIONAL DEPENDENCE; CELLS;

D O I：

10.1109/TRANSDUCERS50396.2021.9495740

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Microfluidic electrical flow cytometry is a popular method to study a wide variety of biological cell properties. Unfortunately, when using coplanar electrodes, this method is sensitive to positional differences of passing particles or cells. In this work we present a novel compensation method to account for the particle position in a coplanar electrode setup using the measured electrical opacity. We demonstrate an accurate size discrimination of 5, 6 and 7 mu m polystyrene beads irrespective of their position using the measured electrical opacity making use of the variation of electrical field strength with height in the channel. Thus, only two electrodes are required, which is favorable for microfluidic devices with size limitations.

引用

页码：1036 / 1039

页数：4

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