A high-throughput screen to identify novel calcineurin inhibitors

被引：4
|
作者：
Margassery, Lekha Menon [1 ,2 ]
Kennedy, Jonathan [1 ,2 ]
O'Gara, Fergal [1 ,2 ,3 ]
Dobson, Alan D. [1 ,2 ]
Morrissey, John P. [1 ,2 ]
机构：
[1] Univ Coll Cork, Ctr Marine Biotechnol, Environm Res Inst, Cork, Ireland
[2] Univ Coll Cork, Dept Microbiol, Cork, Ireland
[3] Univ Coll Cork, BIOMERIT Res Ctr, Cork, Ireland
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 88卷 / 01期
关键词：
Calcineurin inhibitor; High-throughput screen; beta-galactosidase activity; Yeast reporter; CYCLOSPORINE-A; YEAST-CELLS; STRESS; FK-506; FUNGI; ASSAY;
D O I：
10.1016/j.mimet.2011.10.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Calcineurin is a eukaryotic protein phosphatase important for many signalling and developmental processes in cells. Inhibitors of this enzyme are used clinically and there is interest in identifying novel inhibitors for therapeutic applications. This report describes a high-throughput assay that can be used to screen natural or chemical libraries of compounds to identify new calcineurin inhibitors. The microtitre plate assay is based on a yeast reporter strain and was validated with known inhibitors and tested in a pilot screen of bacterial extracts. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：63 / 66
页数：4
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