Morphological and molecular characterization of Phytophthora glovera sp nov from tobacco in Brazil

被引:15
|
作者
Abad, Z. Gloria [2 ]
Ivors, Kelly L. [3 ]
Gallup, Courtney A. [1 ]
Abad, Jorge A. [4 ]
Shew, H. David [1 ]
机构
[1] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA
[2] USDA APHIS PPQ PHP RIPPS, USDA, Mol Diagnost Lab MDL, Beltsville, MD 20705 USA
[3] N Carolina State Univ, Dept Plant Pathol, Mt Hort Crops Res & Extens Ctr MHCREC, Mills River, NC 28759 USA
[4] USDA APHIS PPQ PHP PSPI, USDA, Plant Germplasm Quarantine Program PGQP, Beltsville, MD 20705 USA
关键词
Amarelao; beta-tubulin; ITS; morphology; Oomycetes; Straminipiles; taxonomy; translation elongation factor 1-alpha; NEIGHBOR-JOINING METHOD; PHYLOGENY; SEQUENCES; TROPICALIS; PATHOGEN; CAPSICI; HOSTS; WATER; SOIL;
D O I
10.3852/09-157
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A root rot disease of cultivated tobacco called yellow stunt has been observed in the burley tobacco production areas of Brazil since the early 1990s. Root infecting fungi and straminipiles were isolated from the roots of diseased tobacco plants, including a semi-papillate, homothallic, slow growing Phytophthora species. Pathogenicity trials confirmed that Phytophthora sp. caused root rot and stunting of burley and flue-cured tobaccos. Morphological characteristics of the asexual and sexual stages of this organism did not match any reported Phytophthora species and were very different from the widely known tobacco black shank pathogen P. nicotianae. Phylogenetic analysis based on sequences of the internal transcribed spacer rDNA, beta-tubulin and translation elongation factor 1-alpha regions indicated that this organism represents a previously unreported Phytophthora species that is significantly supported in clack 2 and most closely related to P. capsici. However P. glovera differs from P. capsici in a number of morphological characters, most significantly P. glovera is homothallic and produces both paragynous and amphigynous antheridia while P. capsici is heterothallic and produces only amphigynous antheridia. In this paper we confirmed pathogenicity of this species on tobacco and describe the morphological and molecular characteristics of Phytophthora glovera sp. nov.
引用
收藏
页码:341 / 350
页数:10
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