Magnetic poly(glycerol dimethacrylate) latex particles: synthesis, characterization and cellular interactions

Magnetic poly(glycerol dimethacrylate) (m-poly(GDMA)) latex particles were synthesized by precipitation polymerization in the presence of iron oxide nanoparticles obtained by co-precipitation of Fe2+ and Fe3+ salts. The mean size of iron oxide nanoparticles and m-poly(GDMA) latex particles was determined by dynamic light scattering as 57 and similar to 800 nm, respectively. Vibrating sample magnetometer results showed that iron oxide nanoparticles and m-poly(GDMA) latex particles are superparamagnetic, and their saturation magnetizations are 74.37 and 3.85 emu/g, respectively. MC3T3-E1 preosteoblasts were seeded in the presence of m-poly(GDMA) latex particles. Through the magnetic particles, magnetic field was applied onto the cells by an appropriate magnet (4500 G). Cell-particle interactions were observed by optical microscope and scanning electron microscope. Mitochondrial activities of cells was measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyl tetrazolium bromide test. The results show that the presence of m-poly(GDMA) particles in the culture medium did not affect the proliferation behaviour of MC3T3 cells and no toxicity against to L929 fibroblastic cell proliferation was observed when the particle concentration is 100 pg per cell.