Interaction between TRPV1-expressing neurons in the hypothalamus

被引：22

作者：

Molinas, Adrien T. R. ^{[1
]}

Desmoulins, Lucie D. ^{[1
]}

Hamling, Brooke, V ^{[1
,2
]}

Butcher, Sierra M. ^{[1
,2
]}

Anwar, Imran J. ^{[1
]}

Miyata, Kayoko ^{[1
]}

Enix, Courtney L. ^{[1
]}

Dugas, Courtney M. ^{[1
]}

Satou, Ryousuke ^{[1
]}

Derbenev, Andrei, V ^{[1
,2
]}

Zsombok, Andrea ^{[1
,2
]}

机构：

[1] Tulane Univ, Sch Med, Dept Physiol, 1430 Tulane Ave,8639, New Orleans, LA 70112 USA

[2] Tulane Univ, Brain Inst, Neurosci Program, New Orleans, LA 70112 USA

来源：

JOURNAL OF NEUROPHYSIOLOGY | 2019年 / 121卷 / 01期

基金：

美国国家卫生研究院;

关键词：

capsaicin; dorsomedial hypothalamic nucleus; paraventricular nucleus of the hypothalamus; patch-clamp; TRPV1; RECEPTOR POTENTIAL VANILLOID-1; LIVER-RELATED NEURONS; N-TERMINAL VARIANT; GLUTAMATE RELEASE; PARAVENTRICULAR NUCLEUS; INSULIN-SECRETION; TRPV1; ACTIVATION; TYPE-1; RAT;

D O I：

10.1152/jn.00004.2018

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Transient receptor potential vanilloid type 1 (TRPV1) is a ligand-gated ion channel expressed in the peripheral and central nervous systems. TRPV1-dependent mechanisms take part in a wide range of physiological and pathophysiological pathways including the regulation of homeostatic functions. TRPV1 expression in the hypothalamus has been described as well as evidence that TRPV1-dependent excitatory inputs to hypothalamic preautonomic neurons are diminished in diabetic conditions. Here we aimed to determine the functional expression of TRPV1 in two hypothalamic nuclei known to be involved in the central control of metabolism and to test the hypothesis that TRPV1-expressing neurons receive TRPV1-expressing inputs. A mouse model (TRPV1(Cre/tdTom)) was generated to identify TRPV1-expressing cells and determine the cellular properties of TRPV1-expressing neurons in adult mice. Our study demonstrated the functional expression of TRPV1 in the dorsomedial hypothalamic nucleus and paraventricular nucleus in adult mice. Our findings revealed that a subset of TRPV1(Cre/tdTom) neurons receive TRPV1-expressing excitatory inputs, indicating direct interaction between TRPV1-expressing neurons. In addition, astrocytes likely play a role in the modulation of TRPV1-expressing neurons. In summary, this study identified specific hypothalamic regions where TRPV1 is expressed and functional in adult mice and the existence of direct connections between TRPV1(Cre/tdTom) neurons. NEW & NOTEWORTHY Transient receptor potential vanilloid type 1 (TRPV1) is expressed in the hypothalamus, and TRPV1-dependent regulation of preautonomic neurons is decreased in hyperglycemic conditions. Our study demonstrated functional expression of TRPV1 in two hypothalamic nuclei involved in the control of energy homeostasis. Our results also revealed that a subset of TRPV1-expressing neurons receive TRPV1-expressing excitatory inputs. These findings suggest direct interaction between TRPV1-expressing neurons.

引用

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页码：140 / 151

页数：12

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