Protective Role of Transient Pore Openings in Calcium Handling by Cardiac Mitochondria

被引:60
|
作者
Korge, Paavo
Yang, Ling
Yang, Jun-Hai
Wang, Yibin
Qu, Zhilin
Weiss, James N. [1 ]
机构
[1] Univ Calif Los Angeles, Div Cardiol, David Geffen Sch Med, Cardiovasc Res Lab,Dept Med Cardiol, Los Angeles, CA 90095 USA
基金
美国国家卫生研究院;
关键词
PERMEABILITY TRANSITION PORE; CELL-DEATH; MEMBRANE; CA2+; ISCHEMIA; CHANNELS; RELEASE;
D O I
10.1074/jbc.M111.239921
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Long-lasting mitochondrial permeability transition pore (mPTP) openings damage mitochondria, but transient mPTP openings protect against chronic cardiac stress. To probe the mechanism, we subjected isolated cardiac mitochondria to gradual Ca2+ loading, which, in the absence of BSA, induced long-lasting mPTP opening, causing matrix depolarization. However, with BSA present to mimic cytoplasmic fatty acid-binding proteins, the mitochondrial population remained polarized and functional, even after matrix Ca2+ release caused an extramitochondrial free [Ca2+] increase to >10 mu M, unless mPTP openings were inhibited. These findings could be explained by asynchronous transient mPTP openings allowing individual mitochondria to depolarize long enough to flush accumulated matrix Ca2+ and then to repolarize rapidly after pore closure. Because subsequent matrix Ca2+ reuptake via the Ca2+ uniporter is estimated to be > 100-fold slower than matrix Ca2+ release via mPTP, only a tiny fraction of mitochondria (<1%) are depolarized at any given time. Our results show that transient mPTP openings allow cardiac mitochondria to defend themselves collectively against elevated cytoplasmic Ca2+ levels as long as respiratory chain activity is able to balance proton influx with proton pumping. We found that transient mPTP openings also stimulated reactive oxygen species production, which may engage reactive oxygen species-dependent cardioprotective signaling.
引用
收藏
页码:34851 / 34857
页数:7
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