Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein

被引：69
|
作者：
Cortes-Perez, NG
Bermúdez-Humarán, LG
Le Loir, Y
Rodriguez-Padilla, C
Gruss, A
Saucedo-Cárdenas, O
Langella, P
Montes-de-Oca-Luna, R
机构：
[1] INRA, Unite Rech Laitieres & Genet Appl, F-78352 Jouy En Josas, France
[2] Inst Mexicano Seguro Social, Ctr Invest Biomed, Monterrey 64120, NL, Mexico
[3] Univ Autonoma Nuevo Leon, Fac Ciencias Biol, Lab Inmunol & Virol, San Nicolas De Los Garza 66450, NL, Mexico
来源：
FEMS MICROBIOLOGY LETTERS | 2003年 / 229卷 / 01期
关键词：
Lactococcus lactis; cancer; human papillomavirus; vaccine; immunogenic;
D O I：
10.1016/S0378-1097(03)00778-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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收藏
页码：37 / 42
页数：6
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