Effect of Brn-3a deficiency on primary nociceptors in the trigeminal ganglion

被引:10
|
作者
Ichikawa, H
Schulz, S
Höllt, V
Mo, Z
Xiang, M
Sugimoto, T
机构
[1] Okayama Univ, Grad Sch Med & Dent, Dept Oral Funct & Anat, Okayama 7008525, Japan
[2] Okayama Univ, Grad Sch Med & Dent, Biodent Res Ctr, Okayama 7008525, Japan
[3] Otto Von Guericke Univ, Dept Pharmacol & Toxicol, Magdeburg, Germany
[4] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA
[5] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pediat, Piscataway, NJ 08854 USA
关键词
transcription factor; knockout mouse; neuropeptides; capsaicin receptor; trigeminal ganglion; immunohistochemistry;
D O I
10.1016/j.neures.2004.12.014
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Immunohistochemistry for substance P, somatostatin and vanilloid receptor subtype I as well as receptors for somatostatin and opioids was performed on the trigeminal ganglion in wild-type and Brn-3a knockout mice at postnatal day 0. In wild-type mice, the trigeminal ganglion contained abundant substance P-, vanilloid receptor subtype 1-, sst2A receptor- and delta-opioid receptor-immunoreactive neurons, while the ganglion had only a few mu-opioid receptor-immunoreactive neurons. The Brn-3a deficiency had an effect on the cell size but not the number of substance P-immunoreactive neurons. In knockout mice, the proportion of small immunoreactive neurons markedly increased and that of medium- to large-sized immunoreactive ones correspondingly decreased (mean +/- S.D. = 54.7 +/- 29.1 mu m(2), range = 10.9-220.8 mu m(2)) compared to wild-type mice (mean +/- S.D. = 116.6 +/- 58.6 mu m(2), range = 27.3-400.7 mu m(2)). As for vanilloid receptor subtype 1-immunoreactive neurons, the number and cell size was barely affected by the deficiency. On the other hand, the loss of Brn-3a caused a decrease in the number of sst2A receptor- or delta-opioid receptor-immunoreactive neurons (more than 95% reduction) and an increase in the number of muopioid receptor-immunoreactive neurons (9.3-fold increase). Somatostatin-immunoreactive neurons were not detected in the trigeminal ganglion of wild-type or mutant mice at postnatal day 0. The present study suggests that Brn-3a deficiency may have effects on the survival of trigeminal nociceptors and their expression of some neurochemical substances. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
引用
收藏
页码:445 / 451
页数:7
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