Partition coefficients of amino acids, peptides, and enzymes in dextran plus poly(ethylene glycol) plus water aqueous two-phase systems

被引：1
|
作者：
Kakisaka, K
Shindou, T
Ishidao, T
Iwai, Y [1 ]
Arai, Y
机构：
[1] Kyushu Univ, Grad Sch Engn, Dept Chem Syst & Engn, Fukuoka 8128581, Japan
[2] Univ Occupat & Environm Hlth, Sch Hlth Sci, Kitakyushu, Fukuoka 8078555, Japan
来源：
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 1998年 / 31卷 / 06期
关键词：
thermodynamics; partition coefficient; aqueous two-phase system; osmotic virial equation; hydrophilic parameter;
D O I：
10.1252/jcej.31.991
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
Partition coefficients are measured for five amino acids (aspartic acid, asparagine, methionine, cysteine and histidine) and two peptides (glycyl-glycine and hexa-glycine) in dextran + poly(ethylene glycol)+ water aqueous two-phase system. The partition coefficients of the amino acids and peptides are correlated using the osmotic virial equation. The interaction coefficients contained in the equation can be calculated by hydrophilic group parameters. The partition coefficients of alpha-amylase calculated by the osmotic virial equation with the hydrophilic group parameters are in fairly good agreement with the experimental data, though a relatively large discrepancy is shown for beta-amylase.
引用
收藏
页码：991 / 995
页数：5
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