Expression of the Protective Antigen for PEDV in Transgenic Duckweed, Lemna minor

被引：24

作者：

Ko, Suk-Min ^{[1
]}

Sun, Hyeon-Jin ^{[1
]}

Oh, Myung Jin ^{[2
]}

Song, In-Ja ^{[1
]}

Kim, Min-Jae ^{[1
]}

Sin, Hyun-Sook ^{[1
]}

Goh, Chang-Hyo ^{[3
]}

Kim, Yong-Woo ^{[1
]}

Lim, Pyung-Ok ^{[1
,4
]}

Lee, Hyo-Yeon ^{[1
,5
]}

Kim, Suk Weon ^{[2
]}

机构：

[1] Jeju Natl Univ, Subtrop Hort Res Inst, Cheju 690756, South Korea

[2] Korea Res Inst Biosci & Biotechnol, Biol Resource Ctr, Taejon 305806, South Korea

[3] Jeju Natl Univ, Res Inst Basic Sci, Cheju 690756, South Korea

[4] Jeju Natl Univ, Dept Sci Educ, Cheju 690756, South Korea

[5] Jeju Natl Univ, Fac Biotechnol, Cheju 690756, South Korea

来源：

HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY | 2011年 / 52卷 / 05期

关键词：

aquatic plant; porcine diarrhea; spike protein; transformation; vaccine; SYNTHETIC NEUTRALIZING EPITOPE; EPIDEMIC DIARRHEA VIRUS; TOBACCO PLANTS; PHARMACEUTICALS; TRANSFORMATION;

D O I：

10.1007/s13580-011-0007-x

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

引用

页码：511 / 515

页数：5

共 50 条

[31] NITROGEN AND PHOSPHOROUS REMOVAL FROM LEACHATE BY DUCKWEED (Lemna minor)
Iqbal, Jamshaid
Baig, Muhammad Anwar
ENVIRONMENT PROTECTION ENGINEERING, 2017, 43 (04): : 123 - 134
[32] Flumequine uptake and the aquatic duckweed, Lemna minor L.
Cascone, A
Forni, C
Migliore, L
WATER AIR AND SOIL POLLUTION, 2004, 156 (1-4): : 241 - 249
[33] Enhancement of anaerobic digestion using duckweed (Lemna minor) enriched with iron
Clark, PB
Hillman, PF
JOURNAL OF THE CHARTERED INSTITUTION OF WATER AND ENVIRONMENTAL MANAGEMENT, 1996, 10 (02): : 92 - 95
[34] Magnetic and electric field accelerate Phytoextraction of copper Lemna minor duckweed
Politaeva, Natalia
Badenko, Vladimir
PLOS ONE, 2021, 16 (08):
[35] Enzymatic saccharification of duckweed (Lemna minor) biomass without thermophysical pretreatment
Zhao, X.
Elliston, A.
Collins, S. R. A.
Moates, G. K.
Coleman, M. J.
Waldron, K. W.
BIOMASS & BIOENERGY, 2012, 47 : 354 - 361
[36] Relative sensitivity of duckweed Lemna minor and six algae to seven herbicides
Ueda, Koji
Nagai, Takashi
JOURNAL OF PESTICIDE SCIENCE, 2021, 46 (03) : 267 - 273
[37] Physiological response of duckweed (Lemna minor L.) to herbicide norflurazon
Tkalec, M
Perincic, Z
Vidakovic-Cifrek, Z
Pevalek-Kozlina, B
Regula, I
PERIODICUM BIOLOGORUM, 2003, 105 (03) : 269 - 274
[38] The distribution of lead in duckweed (Lemna minor L.) root tip
Samardakiewicz, S
Wozny, A
PLANT AND SOIL, 2000, 226 (01) : 107 - 111
[39] NEW METHOD FOR DETERMINING EFFLUENT TOXICITY USING DUCKWEED (LEMNA, MINOR)
TARALDSEN, JE
NORBERGKING, TJ
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1990, 9 (06) : 761 - 767
[40] Toxic Responses of Palladium Accumulation in Duckweed (Lemna minor): Determination of Biomarkers
Jmii, Souleimen
Dewez, David
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2021, 40 (06) : 1630 - 1638

← 1 2 3 4 5 →