Treatment of HIV-infected patients with Gc protein-derived macrophage activating factor (GcMAF) eradicates HIV-infection

Gc protein is the precursor for the principal macrophage activating factor (MAF). The MAF precursor activity of serum Gc protein of HIV-infected patients was lost or reduced because Gc protein is deglycosylated by serum alpha-N-acetylgalactosaminidase (Nagalase) secreted from HIV-infected cells. Nagalase was found to be the intrinsic component of gp120. Thus, serum Nagalase activity is the sum of enzyme activities expressed in both HIV virions and envelope proteins. These Nagalase carriers are complexed with patient anti-HIV IgG. Stepwise treatment of purified Gc protein with immobilized beta-galactosidase and sialidase generated the most potent MAF (GcMAF) that can activate macrophages to develop a large amount of Fc-receptors. Less than 18 weekly administrations of 100 ng GcMAF for fourteen HIV-infected patients eradicated HIV-infection.