Comprehensive Transcriptomic Analysis of Auxin Responses in Submerged Rice Coleoptile Growth

被引:18
|
作者
Wu, Yu-Sian [1 ]
Yang, Chin-Ying [1 ,2 ]
机构
[1] Natl Chung Hsing Univ, Dept Agron, Taichung 40227, Taiwan
[2] Pervas AI Res PAIR Labs, Hsinchu 30010, Taiwan
关键词
rice coleoptile; transcriptomic analysis; submergence; auxin signaling; hormone; ABSCISIC-ACID; BACTERIAL-BLIGHT; GENE-EXPRESSION; ARABIDOPSIS; RESISTANCE; PATHWAYS; ETHYLENE; DORMANCY; DROUGHT; STRESS;
D O I
10.3390/ijms21041292
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cultivating rice in wet or water direct seeding systems is simple and time and labor efficient. Rice (Oryza sativa) seeds are a unique cereal that can germinate not only when submerged, but also in anoxic conditions. Many complicated hormone signals interact in submerged seed germination. Ethylene is involved in rice coleoptile elongation, but little is known regarding the role of auxin signaling under submergence. This study demonstrated that the coleoptile is shorter and curlier when submerged with 2,3,5-triiodobenzoic acid (TIBA). In transcriptomic analysis, 3448 of the 31,860 genes were upregulated, and 4360 genes were downregulated with submergence and TIBA treatment. The Gene Ontology function classification results demonstrated that upregulated differentially expressed genes (DEGs) were mainly involved in redox, stress, and signal transduction, whereas the down-regulated DEGs were mainly involved in RNA transcription, stress, and development. Furthermore, auxin signaling involved in the carbohydrate metabolism pathway was demonstrated while using transcriptomic analysis and confirmed in a quantitative real-time polymerase chain reaction. In addition, the transcript levels of development-related genes and mitochondria-electron- transport-related genes were regulated by auxin signaling under submergence. Auxin signaling was not only involved in regulating rice coleoptile elongation and development, but also regulated secondary metabolism, carbohydrate metabolism, and mitochondria electron transport under submergence. Our results presented that auxin signaling plays an important role during rice coleoptile elongation upon the submergence condition and improving the advance of research of direct rice seeding system.
引用
收藏
页数:19
相关论文
共 50 条
  • [21] Elongation growth in the absence of oxygen: The rice coleoptile
    Bertani, A
    Brambilla, I
    Mapelli, S
    Reggiani, R
    RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, 1997, 44 (04) : 543 - 547
  • [22] Vital contribution of brassinosteroids to hypoxia-stimulated coleoptile elongation in submerged rice
    Sun, Binbin
    Meng, Wenjing
    Yin, Wenchao
    Niu, Mei
    Liu, Dapu
    Liu, Jihong
    Zhang, Xiaoxing
    Dong, Nana
    Yang, Yanzhao
    Li, Xicheng
    Geng, Simeng
    Zhang, Haiyan
    Tong, Hongning
    CROP JOURNAL, 2024, 12 (05): : 1379 - 1390
  • [23] Vital contribution of brassinosteroids to hypoxia-stimulated coleoptile elongation in submerged rice
    Binbin Sun
    Wenjing Meng
    Wenchao Yin
    Mei Niu
    Dapu Liu
    Jihong Liu
    Xiaoxing Zhang
    Nana Dong
    Yanzhao Yang
    Xicheng Li
    Simeng Geng
    Haiyan Zhang
    Hongning Tong
    The Crop Journal, 2024, 12 (05) : 1379 - 1390
  • [24] ROLE OF AUXIN IN GROWTH OF INHIBITOR-TREATED OAT COLEOPTILE TISSUE
    ORDIN, L
    GARBER, MJ
    SKOE, BP
    ROLLE, G
    PHYSIOLOGIA PLANTARUM, 1966, 19 (04) : 937 - &
  • [25] EFFECTS OF GIBBERELLIC ACID ON AUXIN FORMATION + GROWTH OF TIP OF AVENA COLEOPTILE
    SASTRY, KSK
    MUIR, RM
    PLANT PHYSIOLOGY, 1964, 39 : R27 - &
  • [26] Dependence of the Avena coleoptile growth-rate on the previous auxin supply
    Schneider, CL
    AMERICAN JOURNAL OF BOTANY, 1940, 27 (09) : 711 - 718
  • [27] MINERAL NUTRITION AND AUXIN-INDUCED GROWTH OF TRITICUM COLEOPTILE SECTIONS
    BURSTROM, HG
    PLANT AND CELL PHYSIOLOGY, 1973, 14 (05) : 941 - 951
  • [28] THE GROWTH-TIME RELATIONSHIPS OF THE AUXIN-INDUCED GROWTH IN AVENA COLEOPTILE SECTIONS
    BONNER, J
    FOSTER, RJ
    JOURNAL OF EXPERIMENTAL BOTANY, 1955, 6 (17) : 293 - 302
  • [30] STEADY STATE GROWTH OF AVENA COLEOPTILE SECTIONS IN HIGH AUXIN CONCENTRATIONS
    KEFFORD, NP
    BONNER, J
    PLANT PHYSIOLOGY, 1961, 36 (03) : 323 - &