A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells

被引：26
|
作者：
Zhang, Zijian [1 ]
Lv, Ting [1 ]
Tao, Binbin [2 ]
Wen, Zhenfu [1 ]
Xu, Yongqian [1 ]
Li, Hongjuan [1 ]
Liu, Fengyu [3 ]
Sun, Shiguo [1 ]
机构：
[1] Northwest A&F Univ, Coll Chem & Pharm, Shaanxi Key Lab Nat Prod & Chem Biol, Xinong Rd 22, Yangling 712100, Shaanxi, Peoples R China
[2] Zhejiang Runtu Co Ltd, 1 Bldg Wealth Sq,1009 Shimin Ave, Shangyu City 312368, Zhejiang, Peoples R China
[3] Dalian Univ Technol, Sch Chem, State Key Lab Fine Chem, 2 Linggong Rd, Dalian 116023, Peoples R China
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2020年 / 28卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Nitroreductase (NTR); 1,8-Naphthalimide; Escherichia coli (E. coli); Staphylococcus aureus. (S. aureus); TURN-ON PROBE; ESCHERICHIA-COLI; LIVING CELLS; REDUCTION; OXIDE; IONS;
D O I：
10.1016/j.bmc.2019.115280
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A nitroreductase (NTR) responsive fluorescent probe, Na-NO2, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized. Na-NO2 showed remarkable fluorescence "turn-on" signal in the presence of NTR under DMSO/H2O (1:19, v/v) buffered with PBS (pH = 7) solution in the presence of NADH (300 mu M). Furthermore, the probe has a low detection limit down to 3.4 ng/mL and it is very sensitive towards the NTR in Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), normal and tumor cells such as HL-7702, HepG-2 and MCF-7.
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页数：6
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