A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells

被引：26

作者：

Zhang, Zijian ^{[1
]}

Lv, Ting ^{[1
]}

Tao, Binbin ^{[2
]}

Wen, Zhenfu ^{[1
]}

Xu, Yongqian ^{[1
]}

Li, Hongjuan ^{[1
]}

Liu, Fengyu ^{[3
]}

Sun, Shiguo ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Chem & Pharm, Shaanxi Key Lab Nat Prod & Chem Biol, Xinong Rd 22, Yangling 712100, Shaanxi, Peoples R China

[2] Zhejiang Runtu Co Ltd, 1 Bldg Wealth Sq,1009 Shimin Ave, Shangyu City 312368, Zhejiang, Peoples R China

[3] Dalian Univ Technol, Sch Chem, State Key Lab Fine Chem, 2 Linggong Rd, Dalian 116023, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2020年 / 28卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Nitroreductase (NTR); 1,8-Naphthalimide; Escherichia coli (E. coli); Staphylococcus aureus. (S. aureus); TURN-ON PROBE; ESCHERICHIA-COLI; LIVING CELLS; REDUCTION; OXIDE; IONS;

D O I：

10.1016/j.bmc.2019.115280

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A nitroreductase (NTR) responsive fluorescent probe, Na-NO2, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized. Na-NO2 showed remarkable fluorescence "turn-on" signal in the presence of NTR under DMSO/H2O (1:19, v/v) buffered with PBS (pH = 7) solution in the presence of NADH (300 mu M). Furthermore, the probe has a low detection limit down to 3.4 ng/mL and it is very sensitive towards the NTR in Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), normal and tumor cells such as HL-7702, HepG-2 and MCF-7.

引用

页数：6

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