Gener cloning and expression characterization of a novel JcBCCPII gene from Jatropha curcas

In the present study, jatropha JcBCCPII gene was cloned and characterized. This novel gene was deposited into NCBI (KC875846). The results of cDNA sequences of the JcBCCPII gene demonstrated that it is a gene with a complete open reading frame of 642 bp encoding a JcBCCPII protein of 213 amino acids. Similarly comparisons for amino acid sequences reveal that the JcBCCPII protein has high homology with the BCCPII proteins of six other species. The phylogenetic tree analysis revealed that the JcBCCPII protein has a closer genetic relationship with the JcBCCPII protein of Ricinus communis and Populus trichocarpa than with those of Vitis vinifera, Glycine max, Lotus japonicas and Arabidopsis thaliana. Real-time PCR analysis shows that JcBCCPII gene is expressed in various tissues but at different levels. The expression levels of this gene are highly expressed in leaf, flower and seed, moderately expressed in stem and pericarp, weakly expressed in root. The JcBCCPII gene displayed a bell-shaped pattern of expression which had peak expression at 30 and 40 DAF but low at the first two stages of seed development, i.e. 10 and 20 DAP. The JcBCCPII expression can be induced by MeJA and BRs. These data provide a foundation for further insight into this gene.