S-allele genotypes of apple pollenizers, cultivars and lineages including those resistant to scab

We examined S-allele genotypes of ten apple cultivars and species to determine their possible usefulness as pollenizers for all apple cultivars. 'Dolgo' did not contain any known S-RNases encoded at the S-locus, suggesting its possible usefulness as a pollenizer for almost all apple cultivars. We also identified and confirmed the S-allele genotypes of 18 apple cultivars by polymerase chain reaction (PCR)-digestion analysis. The S-genotype of 'Kiou' (S1S7), 'Korei' (S3S28), 'Korin' (S1S9),. 'Kotoku' (S1S28), 'Kyokkou (S7S25), 'Lobo' (S1S7), 'Mahe 7' (S2S7), 'Mellow' (S2S3), 'Takahara' (S3S9) and 'Warabi' (S9S28) were confirmed by pollination results. These cultivars seemed not to have, originated from the expected seed or pollen parents or, in the case of 'Lobo', might have been mislabelled. Finally, we identified the S-allele genotypes of 'Prima' (S2S10), 'Querina' (S3S9) and 'Yoko' x 'Prima' (S3S10), which are resistant to scab.