SDF1-Induced Antagonism of Axonal Repulsion Requires Multiple G-Protein Coupled Signaling Components That Work in Parallel

被引：7

作者：

Twery, E. Naomi ^{[1
]}

Raper, Jonathan A. ^{[2
]}

机构：

[1] Univ Penn, Sch Med, Neurosci Grad Grp, Philadelphia, PA 19104 USA

[2] Univ Penn, Sch Med, Dept Neurosci, Philadelphia, PA 19104 USA

来源：

PLOS ONE | 2011年 / 6卷 / 04期

基金：

美国国家卫生研究院;

关键词：

ALPHA-SUBUNIT; CALCIUM MOBILIZATION; PHOSPHOLIPASE-C; IN-VIVO; CELLS; RECEPTORS; CHEMOKINE; REPELLENTS; ACTIVATION; EXPRESSION;

D O I：

10.1371/journal.pone.0018896

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

SDF1 reduces the responsiveness of axonal growth cones to repellent guidance cues in a pertussis-toxin-sensitive, cAMP-dependent manner. Here, we show that SDF1's antirepellent effect can be blocked in embryonic chick dorsal root ganglia (DRGs) by expression of peptides or proteins inhibiting either G alpha(i), G alpha(q), or G beta gamma. SDF1 antirepellent activity is also blocked by pharmacological inhibition of PLC, a common effector protein for G alpha(q). We also show that SDF1 antirepellent activity can be mimicked by overexpression of constitutively active G alpha(i), G alpha(q), or G alpha(s). These results suggest a model in which multiple G protein components cooperate to produce the cAMP levels required for SDF1 antirepellent activity.

引用

页数：8

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