Talin1 phosphorylation activates β1 integrins: a novel mechanism to promote prostate cancer bone metastasis

被引:96
|
作者
Jin, J-K [1 ,2 ]
Tien, P-C [1 ]
Cheng, C-J [3 ]
Song, J. H. [1 ]
Huang, C. [4 ]
Lin, S-H [1 ,2 ,5 ]
Gallick, G. E. [1 ,2 ]
机构
[1] Univ Texas MD Anderson Canc Ctr, David H Koch Ctr Appl Res Genitourinary Canc, Dept Genitourinary Med Oncol, Unit 18 4, Houston, TX 77030 USA
[2] Univ Texas Houston, Grad Sch Biomed Sci, Program Canc Metastasis, Houston, TX USA
[3] Taipei Med Univ, Dept Pathol, Coll Med, Taipei, Taiwan
[4] Univ Kentucky, Dept Mol & Biomed Pharmacol, Lexington, KY USA
[5] Univ Texas MD Anderson Canc Ctr, David H Koch Ctr Appl Res Genitourinary Canc, Dept Translat Mol Pathol, Unit 89, Houston, TX 77030 USA
基金
美国国家卫生研究院;
关键词
INTEGRIN EXPRESSION; BINDING; PROGRESSION; ADHESION; CARCINOMA; SUBUNIT; DOMAIN; TAILS; SITES; CELLS;
D O I
10.1038/onc.2014.116
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Talins are adaptor proteins that regulate focal adhesion signaling by conjugating integrins to the cytoskeleton. Talins directly bind integrins and are essential for integrin activation. We previously showed that beta 1 integrins are activated in metastatic prostate cancer (PCa) cells, increasing PCa metastasis to lymph nodes and bone. However, how beta 1 integrins are activated in PCa cells is unknown. In this study, we identified a novel mechanism of beta 1 integrin activation. Using knockdown experiments, we first demonstrated that talin1, but not talin2, is important in beta 1 integrin activation. We next showed that talin1 S425 phosphorylation, but not total talin1 expression, correlates with metastatic potential of PCa cells. Expressing a non-phosphorylatable mutant, talin1(S425A), in talin1-silenced PC3-MM2 and C4-2B4 PCa cells, decreased activation of beta 1 integrins, integrin-mediated adhesion, motility and increased the sensitivity of the cells to anoikis. In contrast, reexpression of the phosphorylation-mimicking mutant talin1(S425D) led to increased beta 1 integrin activation and generated biologic effects opposite to talin1(S425A) expression. In the highly metastatic PC3-MM2 cells, expression of a non-phosphorylatable mutant, talin1(S425A), in talin1-silenced PC3-MM2 cells, abolished their ability to colonize in the bone following intracardiac injection, while reexpression of phosphorylation-mimicking mutant talin1(S425D) restored their ability to metastasize to bone. Immunohistochemical staining demonstrated that talin S425 phosphorylation is significantly increased in human bone metastases when compared with normal tissues, primary tumors or lymph node metastases. We further showed that p35 expression, an activator of Cdk5, and Cdk5 activity were increased in metastatic tumor cells, and that Cdk5 kinase activity is responsible for talin1 phosphorylation and subsequent beta 1 integrin activation. Together, our study reveals Cdk5-mediated phosphorylation of talin1 leading to beta 1 integrin activation is a novel mechanism that increases metastatic potential of PCa cells.
引用
收藏
页码:1811 / 1821
页数:11
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