In vitro selection for enzymatic activity:: A model study using adenylate cyclase

被引：8
|
作者：
Strobel, H
Ladant, D
Jestin, JL
机构：
[1] Inst Pasteur, Unite Chim Organ, F-75724 Paris 15, France
[2] Inst Pasteur, Unite Postulante Biochim Interact Macromol, Dept Biol Struct & Chim, Paris, France
来源：
JOURNAL OF MOLECULAR BIOLOGY | 2003年 / 332卷 / 01期
关键词：
enzyme catalysis; molecular evolution; gene technology; phage display; lyase;
D O I：
10.1016/S0022-2836(03)00920-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An in vitro enzyme selection that can, in principle, be generalised to most chemical reactions, is described. It makes use of filamentous phage display and of a tailor-made antibody fragment directed against the reaction product. The conversion of ATP into 3',5'-cyclic AMP catalysed by Bordetella pertussis adenylate cyclase is taken as an example. (C) 2003 Elsevier Ltd. All rights reserved.
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页码：1 / 7
页数：7
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