Current advances and strategies towards fully automated sample preparation for regulated LC-MS/MS bioanalysis

被引：27

作者：

Zheng, Naiyu ^{[1
]}

Jiang, Hao ^{[1
]}

Zeng, Jianing ^{[1
]}

机构：

[1] Bristol Myers Squibb Co, Analyt & Bioanalyt Dev, Princeton, NJ 08540 USA

来源：

BIOANALYSIS | 2014年 / 6卷 / 18期

关键词：

SOLID-PHASE EXTRACTION; MASS-SPECTROMETRY METHOD; SUPPORTED LIQUID EXTRACTION; HIGH-THROUGHPUT BIOANALYSIS; HUMAN PLASMA; QUANTITATIVE-DETERMINATION; PROTEIN PRECIPITATION; ESTERASE INHIBITORS; HUMAN SERUM; PHOSPHOLIPIDS;

D O I：

10.4155/bio.14.161

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Robotic liquid handlers (RLHs) have been widely used in automated sample preparation for liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalysis. Automated sample preparation for regulated bioanalysis offers significantly higher assay efficiency, better data quality and potential bioanalytical cost-savings. For RLHs that are used for regulated bioanalysis, there are additional requirements, including 21 CFR Part 11 compliance, software validation, system qualification, calibration verification and proper maintenance. This article reviews recent advances in automated sample preparation for regulated bioanalysis in the last 5 years. Specifically, it covers the following aspects: regulated bioanalysis requirements, recent advances in automation hardware and software development, sample extraction workflow simplification, strategies towards fully automated sample extraction, and best practices in automated sample preparation for regulated bioanalysis.

引用

页码：2441 / 2459

页数：19

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