Evaluation of the interaction between β-cyclodextrin and psychotropic drugs by surface plasmon resonance assay with a Biacore® system

被引：17

作者：

Kobayashi, Hiroaki ^{[1
]}

Endo, Tomohiro ^{[1
]}

Ogawa, Noriko ^{[1
]}

Nagase, Hiromasa ^{[1
]}

Iwata, Mariko ^{[1
]}

Ueda, Haruhisa ^{[1
]}

机构：

[1] Hoshi Univ, Dept Phys Chem, Shinagawa Ku, Tokyo 1428501, Japan

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2011年 / 54卷 / 01期

关键词：

Cyclodextrins; Surface plasmon resonance assay; Psychotropic drugs; Association constant; Phase-solubility method; Continuous variation method; BINDING;

D O I：

10.1016/j.jpba.2010.08.012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Phase-solubility studies have been used to evaluate the solubilizing effects of cyclodextrins (CDs) on lipophilic, water-insoluble drugs. However, large amounts of CDs and drugs are required to measure solubility by phase-solubility studies. Thus, more efficient approaches to evaluate the interaction of CDs with drugs are needed. Herein we introduce a method that evaluates the interaction between immobilized beta-cyclodextrin and psychotropic drugs by surface plasmon resonance assay with a Biacore (R) system. Association constants and stoichiometries observed were generally consistent with values calculated by traditional methods, such as phase-solubility and continuous variation methods. Results showed that the analytical method using Biacore (R) was suitable to evaluate CD-drug interactions. (c) 2010 Elsevier B.V. All rights reserved.

引用

页码：258 / 263

页数：6

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