A green fluorescent protein-based reporter for protein nuclear import studies in Drosophila cells

被引:5
|
作者
Chan, Wing Man [1 ]
Shaw, Pang Chui [1 ]
Chan, Ho Yin Edwin [1 ]
机构
[1] Chinese Univ Hong Kong, Dept Biochem, Lab Drosophila Res, Mol Biotechnol Program, Shatin, Hong Kong, Peoples R China
关键词
GFP; GST; nuclear localization signal; protein nuclear import;
D O I
10.4161/fly.5320
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Green fluorescent protein-based reporters are commonly used to investigate protein nucleocytoplasmic transport. In this study we developed a novel reporter GFP2-GST which consists of two copies of GFP and one copy of GST, and tested it in two commonly used Drosophila cell lines. The size of the GFP2-GST reporter exceeds the passive diffusion limit across the nuclear pore complexes. It shows an exclusive cytoplasmic localization and displays a restrictive nuclear localization when a nuclear localization signal is appended. This reporter will largely facilitate the characterization and identification of NLS sequences in the fly proteome.
引用
收藏
页码:340 / 342
页数:3
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