Separation of amino acid enantiomers VIA chiral derivatization and non-chiral gas chromatography

被引：39
|
作者：
Bertrand, Marylene [1 ,2 ]
Chabin, Annie [1 ,2 ]
Brack, Andre [1 ,2 ]
Westall, Frances [1 ,2 ]
机构：
[1] CNRS, Ctr Biophys Mol, F-45071 Orleans 2, France
[2] Univ Orleans, F-45067 Orleans 2, France
来源：
JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1180卷 / 1-2期
关键词：
non-chiral capillary GC; derivatization; ethyl chloroformate; 2-chloropropanol; amino acid diastereomers;
D O I：
10.1016/j.chroma.2007.12.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two GC-MS methods for the enantioselective separation of the 20 proteinogenic amino acids are compared. Ethyl chloroformate and 2-chloropropanol were used to derivatize amino acid enantiomers. The diastereomers formed were separated on a non-chiral column by capillary gas chromatography. The separation performances were compared to those obtained when using non-chiral derivatization on a chiral column. (c) 2007 Elsevier B.V. All rights reserved.
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页码：131 / 137
页数：7
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