Extraction and Purification of Lipoxygenase from Soybean Using Aqueous Two-Phase System

被引：21
|
作者：
Lakshmi, M. C. [1 ]
Madhusudhan, M. C. [1 ]
Raghavarao, K. S. M. S. [1 ]
机构：
[1] Cent Food Technol Res Inst, CSIR, Dept Food Engn, Mysore 570020, Karnataka, India
来源：
FOOD AND BIOPROCESS TECHNOLOGY | 2012年 / 5卷 / 01期
关键词：
Lipoxygenase; Aqueous two-phase extraction; Precipitation; Purification; LIQUID-LIQUID-EXTRACTION; PRECIPITATION; BROMELAIN;
D O I：
10.1007/s11947-009-0278-8
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The purification of lipoxygenase in soybeans (richest known source) was attempted by precipitation, using polyethylene glycol, followed by aqueous two-phase extraction (ATPE). The preliminary purification by precipitation was standardized which resulted in two-fold purification. The influence of process parameters such as phase forming salt, molecular weight of phase forming polymer, tie line length, and phase volume ratio on the partitioning behavior of lipoxygenase during ATPE was investigated. The aqueous two-phase system of polyethylene glycol 6000 and ammonium sulfate, with inherent pH of 5.2, was found to be most suitable. The tie line length of 41.25% and volume ratio of 1.7 have resulted in 125% activity recovery with the overall purification factor of 4.38 compared to crude enzyme extract.
引用
收藏
页码：193 / 199
页数：7
相关论文
共 50 条
  • [21] Aqueous two-phase extraction coupled with ultrafiltration for purification of amyloglucosidase
    Tanuja, S
    Srinivas, ND
    Gowthaman, MK
    Raghavarao, KSMS
    BIOPROCESS ENGINEERING, 2000, 23 (01) : 63 - 68
  • [22] Lipoxygenase-1 mass-transfer coefficient in aqueous two-phase system using spray extraction column
    Arsalani, V
    Rostami, K
    Kheirolomoom, A
    INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2005, 44 (19) : 7469 - 7473
  • [23] Liquid–Liquid Extraction of Lipase Using Aqueous Two-Phase System
    K. E. Nandini
    N. K. Rastogi
    Food and Bioprocess Technology, 2011, 4 : 295 - 303
  • [24] Extraction of clavulanic acid using aqueous two-phase micellar system
    de Andrade, Evelyn Guedes
    Cardoso Silva, Marcela de Siqueira
    Haga, Raquel Brandao
    Santos, Valeria Carvalho
    Pessoa, Adalberto, Jr.
    Rangel-Yagui, Carlota de O.
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2011, 58 (02) : 103 - 108
  • [25] Extraction and purification of Polyhydroxyalkanoates (PHAs): application of Thermoseparating aqueous two-phase extraction
    Yoong Kit Leong
    Pau Loke Show
    John Chi-Wei Lan
    Hwei-San Loh
    Yee Jiun Yap
    Tau Chaun Ling
    Journal of Polymer Research, 2017, 24
  • [26] Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueous two-phase system
    Tan, Zhi-jian
    Li, Fen-fang
    Xu, Xue-lei
    BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2013, 36 (08) : 1105 - 1113
  • [27] Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueous two-phase system
    Zhi-jian Tan
    Fen-fang Li
    Xue-lei Xu
    Bioprocess and Biosystems Engineering, 2013, 36 : 1105 - 1113
  • [28] Extraction and purification of Polyhydroxyalkanoates (PHAs): application of Thermoseparating aqueous two-phase extraction
    Leong, Yoong Kit
    Show, Pau Loke
    Lan, John Chi-Wei
    Loh, Hwei-San
    Yap, Yee Jiun
    Ling, Tau Chaun
    JOURNAL OF POLYMER RESEARCH, 2017, 24 (10)
  • [29] Extraction and Purification of Quercitrin, Hyperoside, Rutin, and Afzelin from Zanthoxylum Bungeanum Maxim Leaves Using an Aqueous Two-Phase System
    He, Fengyuan
    Li, Dengwu
    Wang, Dongmei
    Deng, Ming
    JOURNAL OF FOOD SCIENCE, 2016, 81 (07) : C1593 - C1602
  • [30] Purification of a non-enveloped virus using sequential aqueous two-phase extraction
    Nold, Natalie M.
    Kriz, Seth A.
    Waldack, Sheridan
    James, Grace
    Colling, Trisha
    Sarvari, Taravat
    Sharma, Vaishali
    Pohkrel, Alexis
    Burghardt, Ethan
    Joshi, Pratik U.
    Heldt, Caryn L.
    JOURNAL OF CHROMATOGRAPHY A, 2025, 1748
12345