Reusable Enzymatic Fiber Mats for Neurotoxin Remediation in Water

Highly effective and reusable organophosphorus hydrolase (OPH)-loaded fiber mats have been fabricated that are capable of degrading toxic organophosphates (OPs) over a broad range of relevant concentrations (from 8 to 8250 ppm). The inherent fragility of enzymes, a major impediment in their incorporation into technologically relevant materials, was overcome while retaining their high catalytic efficiency, selectivity, and sensitivity via a random heteropolymers (RHP) approach. Kinetic analysis guides the design of polycaprolactone matrix morphology from films to fibers, facilitating substrate diffusion in the material. The RHP OPH fiber mats demonstrate excellent stability and reusability with minimal requirements for storage, retaining over 40% of their initial activity after repeated daily use for three months. Practically, present studies provide valuable guidance toward fabrication of enzyme-based functional materials.