Effects of non-covalent interactions with 5-O-caffeoylquinic acid (chlorogenic acid) on the heat denaturation and solubility of globular proteins

被引:222
|
作者
Prigent, SVE
Gruppen, H
Visser, AJWG
van Koningsveld, GA
de Jong, GAH
Voragen, AGJ [1 ]
机构
[1] TNO WU, Ctr Prot Technol, Wageningen, Netherlands
[2] Univ Wageningen, Food Chem Lab, NL-6700 EV Wageningen, Netherlands
[3] Univ Wageningen, Biochem Lab, NL-6700 ET Wageningen, Netherlands
[4] TNO Nutr & Food Res, Zeist, Netherlands
关键词
phenolic compounds; BSA; lysozyme; alpha-lactalbumin; pH; temperature; ionic strength;
D O I
10.1021/jf021229w
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The non-covalent interactions between the monomeric phenolic compound chlorogenic acid (5-CQA) and bovine serum albumin (BSA), lysozyme, and a-lactalbumin were characterized, and their effect on protein properties was examined. 5-CQA had a low affinity for all three proteins, and these interactions seemed to show a negative cooperativity. 5-COA-BSA binding decreased with increasing temperature, whereas pH (pH 3.0 compared to pH 7.0) and ionic strength had no pronounced effect. At high 5-CQA/protein molar ratios, both the denaturation enthalpy and temperature of BSA increased; however, covalent bonds were created at high temperatures. The presence of 5-CQA had no effect on the solubility of BSA and a-lactalbumin as a function of pH, whereas it decreased lysozyme solubility at alkaline pH due to covalent interactions. These results indicate that the non-covalent interactions with 5-CQA do not have pronounced effects on the functional properties of globular proteins in food systems.
引用
收藏
页码:5088 / 5095
页数:8
相关论文
共 50 条
  • [31] Study of the chlorogenic acid extraction with choline chloride-based deep eutectic solvents and its non-covalent interactions analysis
    Herrera-Beltran, Ilse V.
    Mendez-Torruco, Mauricio
    Matus, Myrna H.
    Dominguez, Zaira
    JOURNAL OF PHYSICAL ORGANIC CHEMISTRY, 2023, 36 (11)
  • [32] Chemical Kinetics of 5-o-Caffeoylquinic Acid in Superheated Steam: Effect of Isomerization on Mate (Ilex paraguariensis) Manufacturing
    Zanoelo, Everton Fernando
    Beninca, Cristina
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (24) : 11564 - 11569
  • [33] Physicochemical Properties, Stability, and Functionality of Non-Covalent Ternary Complexes Fabricated with Pea Protein, Hyaluronic Acid and Chlorogenic Acid
    Fu, Wenfei
    Liu, Fujun
    Zhang, Ronglei
    Zhao, Ru
    He, Yuxin
    Wang, Cuina
    FOODS, 2024, 13 (13)
  • [34] Covalent and non-covalent modification of sunflower protein with chlorogenic acid: Identifying the critical ratios that affect techno-functionality
    Jia, Wanqing
    Sethi, Divjyot Singh
    Goot, Atze Jan van der
    Keppler, Julia K.
    FOOD HYDROCOLLOIDS, 2022, 131
  • [35] Experimental study of chemical kinetics and simulation of 5-O-caffeoylquinic acid oxidation during manufacturing of mate (Ilex Paraquariensis)
    Beninca, Cristina
    Kaskantzis, Georges
    Zanoelo, Everton Fernando
    BIOSYSTEMS ENGINEERING, 2009, 104 (04) : 503 - 509
  • [36] Probing covalent and non-covalent interactions between vanillic acid and starch and their effects on digestibility by solid-state NMR
    Ma, Yunxian
    Chen, Ruixi
    Chen, Zidi
    Wang, Zhipeng
    Chen, Jinfeng
    Zhang, Shenggui
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, 251
  • [37] MODELS OF DENATURATION OF GLOBULAR PROTEINS .2. HYDROPHOBIC INTERACTIONS AND CONFORMATIONAL TRANSITION IN POLYMETHACRYLIC ACID
    ANUFRIEVA, EV
    BIRSHTEIN, TM
    NEKRASOVA, TN
    PTITSYN, OB
    SHEVELEVA, TV
    JOURNAL OF POLYMER SCIENCE PART C-POLYMER SYMPOSIUM, 1968, (16PC): : 3519 - +
  • [38] Synthesis, Characterization, and Non-Covalent Interactions of Palladium(II)-Amino Acid Complexes
    Hobart, David B.
    Berg, Michael A. G.
    Rogers, Hannah M.
    Merola, Joseph S.
    MOLECULES, 2021, 26 (14):
  • [39] Investigating Non-Covalent Interactions of Human Serum Albumin with Doxorubicin and Folic Acid
    Bauer, I. A.
    Dmitrienko, E. V.
    BIOCHEMISTRY MOSCOW-SUPPLEMENT SERIES B-BIOMEDICAL CHEMISTRY, 2024, 18 (03) : 231 - 242
  • [40] NMR structural elucidation of dehydrodimers resulting from oxidation of 5-O-caffeoylquinic acid in an apple juice model solution
    Castillo-Fraire, Claudia Mariana
    Pottier, Sandrine
    Bondon, Arnaud
    Salas, Erika
    Bernillon, Stephane
    Guyot, Sylvain
    Poupard, Pascal
    FOOD CHEMISTRY, 2022, 372