A Genetically Encoded FRET Sensor for Intracellular Heme

被引：62

作者：

Song, Yanqun ^{[1
]}

Yang, Maiyun ^{[1
]}

Wegner, Seraphine V. ^{[1
,2
,3
]}

Zhao, Jingyi ^{[4
]}

Zhu, Rongfeng ^{[5
]}

Wu, Yun ^{[6
]}

He, Chuan ^{[1
,2
,3
,7
]}

Chen, Peng R. ^{[1
,5
,7
]}

机构：

[1] Peking Univ, Beijing Natl Lab Mol Sci, Synthet & Funct Biomol Ctr, Key Lab Bioorgan Chem & Mol Engn,Minist Educ,Coll, Beijing 100871, Peoples R China

[2] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

[3] Univ Chicago, Inst Biophys Dynam, Howard Hughes Med Inst, Chicago, IL 60637 USA

[4] Peking Univ, Sch Chem Biol & Biotechnol, Lab Chem Genom, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China

[5] Peking Tinghua Ctr Life Sci, Beijing, Peoples R China

[6] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

[7] Shanghai Univ E Inst Chem Biol, Shanghai, Peoples R China

来源：

ACS CHEMICAL BIOLOGY | 2015年 / 10卷 / 07期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

FLUORESCENT PROTEIN; OXYGENASE;

D O I：

10.1021/cb5009734

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heme plays pivotal roles in various cellular processes as well as in iron homeostasis in living systems. Here, we report a genetically encoded fluorescence resonance energy transfer (FRET) sensor for selective heme imaging by employing a pair of bacterial heme transfer chaperones as the sensory components. This heme-specific probe allows spatial-temporal visualization of intracellular heme distribution within living cells.

引用

页码：1610 / 1615

页数：6

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