Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood

被引:10
|
作者
Marcinczyk, Natalia [1 ]
Misztal, Tomasz [2 ]
Gromotowicz-Poplawska, Anna [1 ]
Zebrowska, Agnieszka [3 ]
Rusak, Tomasz [2 ]
Radziwon, Piotr [3 ,4 ]
Chabielska, Ewa [1 ]
机构
[1] Med Univ Bialystok, Dept Biopharm, PL-15222 Bialystok, Poland
[2] Med Univ Bialystok, Dept Phys Chem, PL-15089 Bialystok, Poland
[3] Reg Ctr Transfus Med, PL-15950 Bialystok, Poland
[4] Med Univ Bialystok, Dept Haematol, PL-15276 Bialystok, Poland
关键词
PECAM-1; thrombosis; inflammation; platelet; NEUTROPHIL EXTRACELLULAR TRAPS; THROMBUS FORMATION; ASPIRIN; ACTIVATION; PHARMACOKINETICS; HEMOSTASIS; INJURY;
D O I
10.3390/ijms22179611
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In our previous study, we introduced the platelet endothelial cell adhesion molecule 1 (PECAM-1)/thrombus ratio, which is a parameter indicating the proportion of PECAM-1 in laser-induced thrombi in mice. Because PECAM-1 is an antithrombotic molecule, the higher the PECAM-1/thrombus ratio, the less activated the platelets. In this study, we used an extracorporeal model of thrombosis (flow chamber model) to verify its usefulness in the assessment of the PECAM-1/thrombus ratio in animal and human studies. Using the lipopolysaccharide (LPS)-induced inflammation model, we also evaluated whether the PECAM-1/thrombus ratio determined in the flow chamber (without endothelium) differed from that calculated in laser-induced thrombosis (with endothelium). We observed that acetylsalicylic acid (ASA) decreased the area of the thrombus while increasing the PECAM-1/thrombus ratio in healthy mice and humans in a dose-dependent manner. In LPS-treated mice, the PECAM-1/thrombus ratio decreased as the dose of ASA increased in both thrombosis models, but the direction of change in the thrombus area was inconsistent. Our study demonstrates that the PECAM-1/thrombus ratio can more accurately describe the platelet activation status than commonly used parameters such as the thrombus area, and, hence, it can be used in both human and animal studies.
引用
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页数:13
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