"Ultra-high-speed Bionanoscope" for Cell and Microbe Imaging

We are developing an ultra-high-sensitivity and ultra-high-speed imaging system for bioscience, mainly for imaging of microbes with visible light and cells with fluorescence emission. Scarcity of photons is the most serious problem in applications of high-speed imaging to the scientific field. To overcome the problem, the system integrates new technologies consisting of (1) an ultra-high-speed video camera with sub-ten-photon sensitivity with the frame rate of more than 1 mega frames per second, (2) a microscope with highly efficient use of light applicable to various unstained and fluorescence cell observations, and (3) very powerful long-pulse-strobe Xenon lights and lasers for microscopes. Various auxiliary technologies to support utilization of the system are also being developed. One example of them is an efficient video trigger system, which detects a weak signal of a sudden change in a frame under ultra-high-speed imaging by canceling high-frequency fluctuation of illumination light. This paper outlines the system with its preliminary evaluation results.