Selection of reference genes for gene expression studies in astrocytomas

被引：12
|
作者：
Gresner, Sylwia M. [1 ]
Golanska, Ewa [1 ]
Kulczycka-Wojdala, Dominika [2 ]
Jaskolski, Dariusz J. [3 ]
Papierz, Wielislaw [4 ]
Liberski, Pawel P. [1 ]
机构：
[1] Med Univ Lodz, Dept Mol Pathol & Neuropathol, PL-92216 Lodz, Poland
[2] Med Univ Lodz, Dept Oncol Pathol, PL-93509 Lodz, Poland
[3] Med Univ Lodz, Dept Neurosurg, PL-90153 Lodz, Poland
[4] Med Univ Lodz, Dept Pathomorphol, PL-92216 Lodz, Poland
来源：
ANALYTICAL BIOCHEMISTRY | 2011年 / 408卷 / 01期
关键词：
BETA-ACTIN; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; INTERNAL STANDARDS; RNA LEVELS; CLASSIFICATION; PCR;
D O I：
10.1016/j.ab.2010.09.010
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene. The effect of reference gene selection on quantitative real-time polymerase chain reaction data interpretation was demonstrated, normalizing the expression data of a selected gene of interest. Thus, GAPDH may be recommended for data normalization in gene expression studies in astrocytomas. Nevertheless, a preliminary validation of reference gene stability is required prior to every study. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：163 / 165
页数：3
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