Study of heat shock response of human umbilical vein endothelial cells (HUVECs) using cDNA microarray

被引：18
|
作者：
Zhou, M.
Zhang, A.
Lin, B.
Liu, J.
Xu, L. X.
机构：
[1] Shanghai Jiao Tong Univ, Sch Life Sci & Technol, Shanghai 200240, Peoples R China
[2] Shanghai Jiao Tong Univ, Key Lab Syst Biomed, Shanghai 200240, Peoples R China
[3] Inst Syst Biol, Washington, DC USA
来源：
INTERNATIONAL JOURNAL OF HYPERTHERMIA | 2007年 / 23卷 / 03期
基金：
中国国家自然科学基金;
关键词：
endothelial cell; heat treatment; cDNA array; apoptosis; necrosis;
D O I：
10.1080/02656730701295441
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Genetic response of human umbilical vein cells (HLTVECs) to heat shock was studied using gene expression analysis. HUVECs were subjected to heat treatment at 44 degrees C and 55 degrees C for 1h, respectively. Four hours after the treatment, gene expression of the treated cells and control cells (37 degrees C) were profiled using cDNA microarray. Data were analyzed using bioinformatics tools, and the results were verified by real-time quantitative PCR experiment. It has been shown that a large number of genes were regulated indicating global response to heat treatment at the genetic level.
引用
收藏
页码：225 / 258
页数：34
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