Isolation and characterization of new cyclohexylacetic acid-degrading bacteria

被引:13
|
作者
Iwaki, Hiroaki [1 ]
Nakai, Emiko [1 ]
Nakamura, Shota [1 ]
Hasegawa, Yoshie [1 ]
机构
[1] Kansai Univ, Dept Life Sci & Biotechnol, Osaka 5648680, Japan
关键词
D O I
10.1007/s00284-008-9158-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A selected strain, KUA-1, which is the first reported Gram-negative organism capable of growth on cyclohexylacetic acid, was identified as a Cupriavidus metallidurans, based on morphologic and physiologic characteristics and its 16S rRNA gene sequence. Metabolite analysis by HPLC-MS indicated that 1-cyclohexenylacetic acid is an intermediate of cyclohexaneacetic acid metabolism in strain KUA-1.
引用
收藏
页码:107 / 110
页数:4
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