Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenic Escherichia coli in a Galleria mellonella model

被引：49
|
作者：
Ciesielczuk, Holly [1 ,2 ]
Betts, Jonathon [1 ]
Phee, Lynnette [1 ]
Doumith, Michel [2 ]
Hope, Russell [3 ]
Woodford, Neil [1 ,2 ]
Wareham, David W. [1 ]
机构：
[1] Univ London, Barts & London Sch Med & Dent, Blizard Inst, Antimicrobial Res Grp,Ctr Immunol & Infect Dis, London, England
[2] Publ Hlth England, Antimicrobial Resistance & Healthcare Associated, London, England
[3] Publ Hlth England, Healthcare Associated Infect & Antimicrobial Resi, Ctr Infect Dis Surveillance & Control, London, England
来源：
VIRULENCE | 2015年 / 6卷 / 02期
关键词：
ExPEC; Galleria mellonella; resistance; sequence type; virulence; PRODUCE CTX-M; WAX MOTH; KLEBSIELLA-PNEUMONIAE; BETA-LACTAMASES; INFECTION; HOST; SYSTEM; BACTEREMIA; RESISTANCE; DROSOPHILA;
D O I：
10.4161/21505594.2014.988095
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Extra-intestinal pathogenic Escherichia coli (ExPEC) are a significant cause of urinary tract infections and bacteraemia worldwide. Currently no single virulence factor or ExPEC lineage has been identified as the sole contributor to severe extra-intestinal infection and/or urosepsis. Galleria mellonella has recently been established as a simple model for studying the comparative virulence of ExPEC. In this study we investigated the virulence of 40 well-characterized ExPEC strains, in G. mellonella, by measuring mortality (larvae survival), immune recognition/response (melanin production) and cell damage (lactate dehydrogenase production). Although mortality was similar between urinary and bloodstream isolates, it was heightened for community-associated infections, complicated UTIs and urinary-source bacteraemia. Isolates of ST131 and those possessing afa/dra, ompT and serogroup O6 were also associated with heightened virulence.
引用
收藏
页码：145 / 151
页数：7
相关论文
共 50 条
  • [31] Ucl fimbriae regulation and glycan receptor specificity contribute to gut colonisation by extra-intestinal pathogenic Escherichia coli
    Hancock, Steven J.
    Lo, Alvin W.
    Ve, Thomas
    Day, Christopher J.
    Tan, Lendl
    Mendez, Alejandra A.
    Minh-Duy Phan
    Nguyen Thi Khanh Nhu
    Peters, Kate M.
    Richards, Amanda C.
    Fleming, Brittany A.
    Chang, Chyden
    Ngu, Dalton H. Y.
    Forde, Brian M.
    Haselhorst, Thomas
    Goh, Kelvin G. K.
    Beatson, Scott A.
    Jennings, Michael P.
    Mulvey, Matthew A.
    Kobe, Bostjan
    Schembri, Mark A.
    PLOS PATHOGENS, 2022, 18 (06)
  • [32] RcsB-dependent regulation of type VI secretion system in porcine extra-intestinal pathogenic Escherichia coli
    Hu, Linlin
    Yu, Feifei
    Liu, Manli
    Chen, Jing
    Zong, Bingbing
    Zhang, Yanyan
    Chen, Tumei
    Wang, Chenchen
    Zhang, Tongchao
    Zhang, Junli
    Zhu, Yongwei
    Wang, Xiangru
    Chen, Huanchun
    Tan, Chen
    GENE, 2021, 768
  • [33] Molecular Genetic and Functional Analysis of pks-Harboring, Extra-Intestinal Pathogenic Escherichia coli From India
    Suresh, Arya
    Ranjan, Amit
    Jadhav, Savita
    Hussain, Arif
    Shaik, Sabiha
    Alam, Munirul
    Baddam, Ramani
    Wieler, Lothar H.
    Ahmed, Niyaz
    FRONTIERS IN MICROBIOLOGY, 2018, 9
  • [34] Plasmid-mediated fluoroquinolone resistance associated with extra-intestinal Escherichia coli isolates from hospital samples
    Shetty, Shruthi S.
    Deekshit, Vijaya Kumar
    Jazeela, Kadeeja
    Vittal, Rajeshwari
    Rohit, Anusha
    Chakraborty, Anirban
    Karunasagar, Indrani
    INDIAN JOURNAL OF MEDICAL RESEARCH, 2019, 149 (02) : 192 - 198
  • [35] Virulence factors of Escherichia coli, with emphasis on avian pathogenic isolates
    Landman, W. J. M.
    Cornelissen, R. A.
    TIJDSCHRIFT VOOR DIERGENEESKUNDE, 2006, 131 (22) : 822 - 830
  • [36] Galleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates
    Dongmei Shi
    Zhiya Yang
    Wanqing Liao
    Chen Liu
    Liang Zhao
    Huilin Su
    Xiaodong Wang
    Huan Mei
    Min Chen
    Yinggai Song
    Sybren de Hoog
    Shuwen Deng
    IMA Fungus, 15
  • [37] Galleria mellonella in vitro model for chromoblastomycosis shows large differences in virulence between isolates
    Shi, Dongmei
    Yang, Zhiya
    Liao, Wanqing
    Liu, Chen
    Zhao, Liang
    Su, Huilin
    Wang, Xiaodong
    Mei, Huan
    Chen, Min
    Song, Yinggai
    de Hoog, Sybren
    Deng, Shuwen
    IMA FUNGUS, 2024, 15 (01)
  • [38] Effects of single and multiple pathogenicity island deletions on uropathogenic Escherichia coli strain 536 intrinsic extra-intestinal virulence
    Tourret, Jerome
    Diard, Mederic
    Garry, Louis
    Matic, Ivan
    Denamur, Erick
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 300 (07) : 435 - 439
  • [39] Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Isolated in Brazil
    Maluta, Renato Pariz
    Logue, Catherine Mary
    Tiba Casas, Monique Ribeiro
    Meng, Ting
    Lopes Guastalli, Elisabete Aparecida
    Galvao Rojas, Thais Cabrera
    Montelli, Augusto Cezar
    Sadatsune, Terue
    Ramos, Marcelo de Carvalho
    Nolan, Lisa Kay
    da Silveira, Wanderley Dias
    PLOS ONE, 2014, 9 (08):
  • [40] Catecholate siderophore esterases Fes, IroD and IroE are required for salmochelins secretion following utilization, but only IroD contributes to virulence of extra-intestinal pathogenic Escherichia coli
    Caza, Melissa
    Garenaux, Amelie
    Lepine, Francois
    Dozois, Charles M.
    MOLECULAR MICROBIOLOGY, 2015, 97 (04) : 717 - 732
12345