The detection of hepatitis E virus in feces samples of dead pigs in the period from 2011 to 2015 in Slovenia

被引:0
|
作者
Toplak, Ivan [1 ]
Rihtaric, Danijela [1 ]
Barlovic, Smiljka [2 ]
Lainscek, Petra Raspor [3 ]
Kirbis, Andrej [3 ]
机构
[1] Univ Ljubljani, Inst Mikrobiol & Parazitol, Vet Fak, Ljubljana, Slovenia
[2] Univ Ljubljani, Nacl Vet Inst, Enota Murska Sobota, Vet Fak, Ljubljana, Slovenia
[3] Univ Ljubljani, Inst Varno Hrano Krmo & Okolje, Vet Fak, Ljubljana, Slovenia
关键词
Hepatitis E virus; detection; real-time RT-PCR; pig feces; pig;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Between years 2011 and 2015 a total 566 individual feces samples of dead pigs were examined for the detection of nucleic acid of hepatitis E virus (HEV) by real time RTPCR method. In the five-year follow-up period the presence of HEV was demonstrated in an average of 8,83% of feces samples. The lowest percentage of HEV positive samples was detected in year 2013 (4,21%) and the highest in 2014 (12,50%). The cycle threshold values obtained by real time RT-PCR method for positive samples varied from 23 up to 40, reflecting the presence from high to low quantities of HEV in feces. Although HEV does not cause clinical picture of the disease in pigs, the presence of the virus in the feces of dead pigs confirmed that HEV is circulating in Slovenian pig farms. These infections can not be underestimated because of the potential transmission of HEV from pigs to humans.
引用
收藏
页码:154 / 156
页数:3
相关论文
共 50 条
  • [1] The presence of PRRS virus in the period from 2011 to 2016 in the dead pigs in Slovenia
    Toplak, Ivan
    Stukelj, Marina
    Rihtaric, Danijela
    [J]. SLOVENIAN VETERINARY RESEARCH, 2016, 53 : 162 - 164
  • [2] Prevalence and genetic characterization of Hepatitis E virus in paired samples of feces and serum from naturally infected pigs
    Fernandez-Barredo, Salceda
    Galiana, Carolina
    Garcia, Angel
    Gomez-Munoz, Maria Teresa
    [J]. CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 2007, 71 (03): : 236 - 240
  • [3] Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
    Gardinali, Noemi Rovaris
    Barry, Aline Fernandes
    Arellano Otonel, Rodrigo Alejandro
    Alfieri, Alice Fernandes
    Alfieri, Amauri Alcindo
    [J]. MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2012, 107 (07): : 935 - 939
  • [4] A comprehensive study of hepatitis E virus infection in pigs entering a slaughterhouse in Slovenia
    Lainscek, Petra Raspor
    Toplak, Ivan
    Kirbis, Andrej
    [J]. VETERINARY MICROBIOLOGY, 2017, 212 : 52 - 58
  • [5] Detection of hepatitis E virus in slaughtered pigs in Italy
    Barbara Di Martino
    Federica Di Profio
    Vito Martella
    Elisabetta Di Felice
    Cristina E. Di Francesco
    Chiara Ceci
    Fulvio Marsilio
    [J]. Archives of Virology, 2010, 155 : 103 - 106
  • [6] Detection of hepatitis E virus in slaughtered pigs in Italy
    Di Martino, Barbara
    Di Profio, Federica
    Martella, Vito
    Di Felice, Elisabetta
    Di Francesco, Cristina E.
    Ceci, Chiara
    Marsilio, Fulvio
    [J]. ARCHIVES OF VIROLOGY, 2010, 155 (01) : 103 - 106
  • [7] Prevalence of Anti-Hepatitis E Virus Antibodies and First Detection of Hepatitis E Virus in Wild Boar in Slovenia
    Zele, Diana
    Barry, Aline F.
    Hakze-van der Honing, Renate W.
    Vengust, Gorazd
    van der Poel, Wim H. M.
    [J]. VECTOR-BORNE AND ZOONOTIC DISEASES, 2016, 16 (01) : 71 - 74
  • [8] Molecular Detection of Hepatitis E Virus in Sewage Samples
    La Rosa, Giuseppina
    Pourshaban, Manoochehr
    Iaconelli, Marcello
    Vennarucci, Valentina Spuri
    Muscillo, Michele
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (17) : 5870 - 5873
  • [9] IMMUNOHISTOCHEMICAL DETECTION OF HEPATITIS E VIRUS IN NATURALLY AFFECTED PIGS
    Novosel, D.
    Lipej, Z.
    Vojta, A.
    Forgach, P.
    Jungie, A.
    Branovic-Cakanic, K.
    [J]. JOURNAL OF COMPARATIVE PATHOLOGY, 2010, 143 (04) : 355 - 355
  • [10] Molecular detection of Hepatitis E virus in German domestic pigs
    Baechlein, Christine
    Seehusen, Frauke
    Nathues, Heiko
    Beilage, Elisabeth Grosse
    Baumgaertner, Wolfgang
    Grummer, Beatrice
    [J]. BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT, 2013, 126 (1-2): : 25 - 31