Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil

被引:26
|
作者
Gardinali, Noemi Rovaris [1 ]
Barry, Aline Fernandes [1 ]
Arellano Otonel, Rodrigo Alejandro [1 ]
Alfieri, Alice Fernandes [1 ]
Alfieri, Amauri Alcindo [1 ]
机构
[1] Univ Estadual Londrina, Lab Virol Anim, Dept Med Vet Prevent, Londrina, PR, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2012年 / 107卷 / 07期
关键词
hepatitis E virus; swine; slaughterhouse; SWINE; INFECTION; TRANSMISSION; GENOTYPE; FECES; HEV;
D O I
10.1590/S0074-02762012000700016
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Parana, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.
引用
收藏
页码:935 / 939
页数:5
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