Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro

被引：10

作者：

Calvo-Garrido, Javier ^{[1
]}

Winn, Dania ^{[2
]}

Maffezzini, Camilla ^{[1
,5
]}

Wedell, Anna ^{[3
,4
]}

Freyer, Christoph ^{[1
,4
]}

Falk, Anna ^{[2
]}

Wredenberg, Anna ^{[1
,4
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden

[2] Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden

[3] Karolinska Inst, Dept Mol Med & Surg, S-17177 Stockholm, Sweden

[4] Karolinska Univ Hosp, Ctr Inherited Metab Dis, S-17165 Stockholm, Sweden

[5] Ist Sci San Raffaele, Div Neurosci, Stem Cell & Neurogenesis Unit, I-20132 Milan, Italy

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 02期

基金：

瑞典研究理事会;

关键词：

Freyer; Anna Falk; NES cells; neurons; Proliferate; maintain; surface; cell density;

D O I：

10.1016/j.xpro.2021.100528

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012).

引用

页数：21

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