Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro

被引：10
|
作者：
Calvo-Garrido, Javier [1 ]
Winn, Dania [2 ]
Maffezzini, Camilla [1 ,5 ]
Wedell, Anna [3 ,4 ]
Freyer, Christoph [1 ,4 ]
Falk, Anna [2 ]
Wredenberg, Anna [1 ,4 ]
机构：
[1] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
[2] Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden
[3] Karolinska Inst, Dept Mol Med & Surg, S-17177 Stockholm, Sweden
[4] Karolinska Univ Hosp, Ctr Inherited Metab Dis, S-17165 Stockholm, Sweden
[5] Ist Sci San Raffaele, Div Neurosci, Stem Cell & Neurogenesis Unit, I-20132 Milan, Italy
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 02期
基金：
瑞典研究理事会;
关键词：
Freyer; Anna Falk; NES cells; neurons; Proliferate; maintain; surface; cell density;
D O I：
10.1016/j.xpro.2021.100528
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012).
引用
收藏
页数：21
相关论文
共 50 条
  • [1] Antipsychotics promote neural differentiation of human iPS cell-derived neural stem cells
    Asada, Minoru
    Mizutani, Shuki
    Takagi, Masatoshi
    Suzuki, Hidenori
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2016, 480 (04) : 615 - 621
  • [2] Effects of retinoic acids on the differentiation of human embryonic stem cell-derived neural stem cells in vitro
    Kubickova, B.
    Martinkova, S.
    Hilscherova, K.
    TOXICOLOGY LETTERS, 2021, 350 : S137 - S137
  • [3] In Vitro Differentiation of Human iPS Cells into Neural like Cells on a Biomimetic Polyurea
    Hoveizi, Elham
    Ebrahimi-Barough, Somayeh
    Tavakol, Shima
    Sanamiri, Khadije
    MOLECULAR NEUROBIOLOGY, 2017, 54 (01) : 601 - 607
  • [4] In Vitro Differentiation of Human iPS Cells into Neural like Cells on a Biomimetic Polyurea
    Elham Hoveizi
    Somayeh Ebrahimi-Barough
    Shima Tavakol
    Khadije Sanamiri
    Molecular Neurobiology, 2017, 54 : 601 - 607
  • [5] In vitro differentiation of human embryonic neural stem cells
    Nat, R
    Cretoiu, D
    Popescu, LM
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2001, 5 (03): : 324 - 325
  • [6] A Simple Protocol for the Myocardial Differentiation of Human iPS Cells
    Aikawa, Nobuo
    Suzuki, Yui
    Takaba, Katsumi
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2015, 38 (07) : 1070 - 1075
  • [7] Evaluation of the immunogenicity of human iPS cell-derived neural stem/progenitor cells in vitro
    Ozaki, Masahiro
    Iwanami, Akio
    Nagoshi, Narihito
    Kohyama, Jun
    Itakura, Go
    Iwai, Hiroki
    Nishimura, Soraya
    Nishiyama, Yuichiro
    Kawabata, Soya
    Sugai, Keiko
    Iida, Tsuyoshi
    Matsubayashi, Kohei
    Isoda, Miho
    Kashiwagi, Rei
    Toyama, Yoshiaki
    Matsumoto, Morio
    Okano, Hideyuki
    Nakamura, Masaya
    STEM CELL RESEARCH, 2017, 19 : 128 - 138
  • [8] Differentiation of functional human insulin-producing stem cell derived beta cells from iPS cells
    Chimienti, F.
    Kvist, A.
    Forslow, A.
    Eriksson, J.
    Stillemark-Billton, P.
    Sorhede-Winzell, M.
    Wennberg-Huldt, C.
    Andersson, E. -M.
    Eliasson, P.
    Hicks, R.
    Shvartsman, D.
    Melton, D. A.
    Tyrberg, B.
    DIABETOLOGIA, 2016, 59 : S23 - S23
  • [9] In vitro differentiation of human parthenogenetic stem cells into neural lineages
    Isaev, Dmitry A.
    Garitaonandia, Ibon
    Abramihina, Tatiana V.
    Zogovic-Kapsalis, Tatjana
    West, Richard A.
    Semechkin, Andrey Y.
    Mueller, Albrecht M.
    Semechkin, Ruslan A.
    REGENERATIVE MEDICINE, 2012, 7 (01) : 37 - 45
  • [10] Evaluation of human iPS cells by neural differentiation and tumorigenicity
    Okada, Yohei
    Miya, Fuyuki
    Kanemura, Yonehiro
    Sunabori, Takehiko
    Koike, Masato
    Kohda, Kazuhisa
    Yuzaki, Michisuke
    Uchiyama, Yasuo
    Tsunoda, Tatsuhiko
    Yamanaka, Shinya
    Okano, Hideyuki
    NEUROSCIENCE RESEARCH, 2011, 71 : E329 - E330
12345