StAR and steroidogenic enzyme transcriptional regulation in the rat brain: effects of acute alcohol administration

In this study, we showed the presence of steroidogenic acute regulatory protein (StAR) mRNA in the rat brain using Northern blot analysis and revealed its localization using in situ hybridization histochemistry. Although the expression level is less than peripheral steroidogenic organs, the brain has two kinds of StAR transcripts with the same size as peripheral endocrine organs. As expected, StAR mRNA levels were maintained in the brain after gonadectomy and adrenalectomy, which indicates its independent expression. Acute alcohol administration affected the mRNA expression of a variety of steroidogenic enzymes, as well as StAR, in several brain regions, and these changes varied from one region to another. It is suggested that StAR may take part in brain steroidogenesis along with steroidogenic enzymes and that various neurosteroids are synthesized differentially to meet local demands. (C) 2003 Elsevier Science B.V. All rights reserved.