In vitro angiogenic effects of pancreatic bile salt-dependent lipase

Objective - Bile salt- dependent lipase ( BSDL), a lipolytic enzyme secreted in the duodenum by pancreatic acinar cells, has been detected in the serum of all patients and in atheromatous plaque, suggesting its potential implication in vascular pathophysiology. Methods and Results - In vitro pancreatic BSDL evokes human umbilical vein endothelial cell ( HUVEC) proliferation and chemotactic migration. BSDL at mitogen concentration is capable to heal wounded HUVEC monolayer and to promote capillary network formation. HUVEC proliferation depends on the displacement of basic fibroblast growth factor and vascular endothelial growth factor from the extracellular matrix and the activation of extracellular signal- regulated kinases ( ERK1/ 2), p38 mitogen- activated protein kinase, and focal adhesion kinase signaling pathways. Conclusion - For the first time to our knowledge, it is suggested that circulating BSDL could be involved in pathophysiological angiogenesis.