Analysis of sequence variation in Gnathostoma spinigerum mitochondrial DNA by single-strand conformation polymorphism analysis and DNA sequence

被引：15
|
作者：
Ngarmamonpirat, C
Waikagul, J [1 ]
Petmitr, S
Dekumyoy, P
Rojekittikhun, W
Anantapruti, MT
机构：
[1] Mahidol Univ, Fac Trop Med, Dept Helminthol, Bangkok 10400, Thailand
[2] Mahidol Univ, Fac Trop Med, Dept Trop Nutr & Food Sci, Bangkok 10400, Thailand
来源：
PARASITOLOGY INTERNATIONAL | 2005年 / 54卷 / 01期
关键词：
Gnathostoma spinigerum; mitochondrial DNA; cytochrome c oxidase subunit I; polymerase chain reaction-single-strand conformation polymorphism;
D O I：
10.1016/j.parint.2004.12.002
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Morphological variations were observed in the advance third stage larvae of Gnathostoma spinigerum collected from swamp eel (Fluta alba), the second intermediate host. Larvae with typical and three atypical types were chosen for partial cytochrome c oxidase subunit I (COI) gene sequence analysis. A 450 bp polymerase chain reaction product of the COI gene was amplified from mitochondrial DNA. The variations were analyzed by single-strand conformation polymorphism and DNA sequencing. The nucleotide variations of the COI gene in the four types of larvae indicated the presence of an intra-specific variation of mitochondrial DNA in the G. spinigerum population. (C) 2005 Elsevier Ireland Ltd. All rights reserved.
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页码：65 / 68
页数：4
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