Quantitative phase imaging by optimized asymmetric illumination

被引:3
|
作者
Suzuki, Yoshimasa [1 ,2 ]
Odaira, Mayumi [1 ]
Ohde, Hisashi [1 ]
Kawata, Yoshimasa [2 ]
机构
[1] Olympus Corp, Opt Technol R&D Dept, Hachioji, Tokyo 1928512, Japan
[2] Shizuoka Univ, Grad Sch Sci & Technol, Hamamatsu, Shizuoka 4328561, Japan
关键词
HIGH-RESOLUTION; CONTRAST; MICROSCOPY; MORPHOLOGY;
D O I
10.1364/AO.56.007237
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We have presented a simple approach for quantitative phase imaging by optimizing asymmetric illumination of a conventional microscope. With this illumination, the light intensity modulation accompanying refraction at the surface profile of phase objects occurs, and "phase-gradient information" can be derived by detecting it. Two images with phase-gradient information on different axes are converted into the two-dimensional phase distribution of the specimen by introducing the phase-gradient transfer function, which is the intensity change due to refraction by the phase-gradient of a specimen. We experimentally confirm accurate and repeatable performance of our method and demonstrate phase imaging of live cells. (C) 2017 Optical Society of America
引用
收藏
页码:7237 / 7242
页数:6
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